Effects of non-neuronal AChRs on proliferation of splenocytes of mice

Abstract

Abstract: AIM: To examine the effects of non-neu-ronal AChRs on the proliferation of splenocytes.METHODS: The effects of AChRs agonist carbachol on the prolif-eration of mice splenocytes stimulated by concanavalin measured by MTT reduction assay in vitro.RESULTS: The splenocytes responded to carbachol(in the range of 10^-11-10^-9mol·L^-1and 10^-5-10^-4mol·L^-1)byin-creased proliferation which were blocked by the muscarin-ic antagonist atropine(10^-7mol·L^-1).In contrast,car-bachol(10^-7-10^-6mol·L^-1)produced diminished pro-liferation,an effect which were blocked by the nAChRs antagonist mecamylamine(10^-7mol·L^-1).It suggests that mAChRs was sensitive to 10^-11-10^-9mol·L^-1and 10^-5-10^-4mol·L^-1carbachol,resulting in increasing proliferation and nAChRs responses to 10^-7~ 10^-6mol·L^-1carbachol leading to proliferation inhibition.CONCLUSION: Carbachol can elicit two different ef-fects according to different concentrations in splenocytes via non-neuronal mAChRs or nAChRs.

Key words: non-neuronal, ACh receptors, lympho-cytes, proliferation, carbachol

CLC Number:

R965.1

WEN Xiao-ming, ZHANG Ying-li, LIN Xi-min, GUO Shun-xing, WANG Hai. Effects of non-neuronal AChRs on proliferation of splenocytes of mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(11): 1304-1308.

References

1 WesslerI,Kirkpatrick CJ,Racke K.Non-neuronal acetylcho-line,a locally acting molecule,widely distributed in biological system:expression andfunction in human[J].Pharmacol Ther,1998;77:59-79
2 Kawashima K,Fujii T.The lymphocytic cholinergic system and its biological function[J].Life Sci,2003;72:2101-10
3 Fujii T,Tsuchiya T,Yamada S,Fujimoto K,Suzuki T,Kasahara T,et al.Localization and synthesis of acetylcholine in human leukemic T-cell lines[J].J Neurosci Res,1996;44:66-7
4 Fujii T,Yamada S,Watanabe Y,Misama H,Tajima S,Fujimoto K,et al.Induction of choline acetyltrasferase mRNA in human mononuclearleukocytes stimulated by phytohemagglutinin,a T-cell activator[J].J Neuroimmunol,1998;82:101-7
5 Fujii T,Watanabe Y,Inoue T,Kamashima K.Upregulation of mRNA encoding the M 5 muscarinic acetylcholine receptorin hu-man T-and B-lymphocytes during immunological responses[J].Neurochem Res,2003;28:423-9
6 Kawashima K,Fujii T.Extraneuronal cholinergic system in lym-phocytes[J].Pharmacol Ther,2000;86:29-48
7 Karla R,Singh SP,Savage SM,Fujimoto K,Suzuki T,Kasahara T,et al.Effect of cigarette smoke on immune response:Expo-sure to cigarette smoke impairs antigen-mediated signaling in T cells and depletes IP3-sensitive Ca 2+stores[J].J Pharmacol ExPTher,2000;293:166-71
8 Kimura R,Fujii T,Kawashima K.Ca 2+signaling via a7 nicotin-ic acetylcholine receptorin the CEM human leukemic T cell line[J].J Pharm Sci,2003;91:230
9 Richman DP,Arnason BGW.Nicotinic acetylcholine receptor:evidence fora functionally distinct receptoron human lympho-cytes[J].Proc Natl Acad Sci USA,1979;76:4632-5
10 Maslinski W,Kullberg M,Nordstrom O,Bartfai H.Muscarinic receptors and receptor-mediated actions on rat thymocytes[J].J Neuroimmunol,1988;17:265-74
11 Masturzo P,Salmona M,Nordstrom O,Condo S,Ladinsky H.In-tact human lymphocyte membranes respond to muscarinic recep-torstimulation by oxotremorine withmarked changesinmicrovis-cosity and an increase in cyclic GMP[J].FEBS Lett,1985;192:194-8
12 Fujii T,Yamada S,Misawa H,Tajima S,Fujimoto L,Suzuki T,et al.Expression of choline acetyltransferase mRNA and protein in T-lymphocytes[J].Proc Jpn Acad,1995;71:231-5
13 Fujimoto K,MatsuiM,Fujii T,Kawashima K.Decreased acetyl-choline content and choline acetyltransferase mRNA expression in circulating mononuclearleukocytes andlymphoid organs of the spontaneously hypertensive rat[J].Life Sci,2001;69:1629-38

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