Effects of millimeterwave and adriamycin on superoxide dismutase and malondialdehyde of leukemia cell line K562

Abstract

Abstract: AIM: To study the effect of combination millimeterwave with adriamycin on regulating the antioxi-dase system andoxyradical in the cultured cell line system in vitro.METHODS: Leukemic cell line K562 was treated by millimeterwave alone and in combination with adriamycin.Then Intracellularmalondialdehyde(MDA)was analyzed with thiobarbituric acid method,superoxide dismutase (SOD) was analyzed with pyrogallic acid meth-od,and glutathione peroxidase (GSH-Px) was analyzed with improved Hafemanmethod.RESULTS: The combi-nation of millimeterwave and adriamycin had a synergistic effect on inhibiting superoxide dismutase and malondial-dehyde of leukemic cell line K562.The activity of SOD and GSH-Px was decreased and the content of MDA was improved by the combination.CONCLUSION: The combination of millimeterwave and adriamycin shows a synergistic effect on puring leukemic cells in vitro.

Key words: millimeterwave, adriamycin, leuke-mia, superoxide dismutase, malondialdehyde, K562

CLC Number:

R318.03

SUN Gui-ling, JIANG Yue-hua, LAI Sheng-li, YANG Tai-cheng. Effects of millimeterwave and adriamycin on superoxide dismutase and malondialdehyde of leukemia cell line K562[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(12): 1358-1363.

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