Changes of p38 mitogen-activated protein kinase in lung tissue and effects of dexamethasone in asthmatic rats

Abstract

Abstract: AIM: To identify the changes of p38 mi-togen-activated protein kinase (p38MAPK) in lung tissue of asthmatic rats and the effects of dexamethasone on it. METHODS: Ovalbumin (OVA) was injected intraper-itoneally and inhaled to produce the asthmatic model. Twenty ratswere randomly divided into three groups:con-trol group, asthma group and DEX group. The concentra-tion of IL-5 in BALF and the expression of phospho-p38 MAPK in lung tissue were measured by ELISA and West-ern blot, respectively. RESULTS: The expression of phospho-p38 MAPK was up-regulated in the lung of asth-matic rats.The injection of DEX intraperitoneally de-creased the expression of phospho-p38 MAPK and abol-ished the increases of airway resistance, the concentration of IL-5 and the number of eosinophil in BALF. His-topathologic damage of lung tissue was alleviated.There were positive correlations between the expression of phos-pho-p38 MAPK and airway resistance, the concentration of IL-5 and the number of eosinophil in BALF. CONCLUSION: p38 MAPK may play a role in pathological process of asthma. DEX can effectively treat asthma by inhibiting the expression of p38 MAPK.

Key words: asthma, p38 mitogen-activated protein kinase, dexamethasone, interleukine

CLC Number:

R966

HUANG Cui-ping, YANG He-ping, ZHANG Zhen-xiang, XU Yong-jian. Changes of p38 mitogen-activated protein kinase in lung tissue and effects of dexamethasone in asthmatic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(5): 555-558.

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