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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2006, Vol. 11 ›› Issue (10): 1102-1105.

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A simple and repeatable orthotopic model of the A549 cell line in nude mice

KANG Kai, MA Bing-liang1   

  1. Beijing Starvax Biotechnology Incorporation, Life Park Road, Beijing 102206 , China;
    1Department of Pharmacy ,College of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203 , China
  • Received:2006-05-30 Accepted:2006-06-14 Online:2006-10-26 Published:2020-11-05
  • About author:KANG Kai,male, master of pharmacology, majoring in onco-pharmacology.Tel:010-80728608-208 ;13466369829 E-mail:bigoose 1979 @eyou.com

Abstract: AIM: To establish a simple and repeatable orthotopic animal model of the A549 cell line.METHODS: A549 cells in matrigel were inoculated into left lung or right flank of nude mouse.The growth pattern of A549 tumors and the chemotherapeutic efficacy of carboplatinum were studied.RESULTS: Tumors generated and dispersed widespreadly to contralateral lung.Tumors in lung were less susceptible to carboplatinum than those growing in flank.CONCLUSION: A simple and repeatable orthotopic animal model of the A549 cell line was presented,this allows researchers to evaluate novel compounds and new drugs for treatment of human lung cancer.

Key words: A549 cell line, orthotopic model, nude mice

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