Abstract: AIM: To investigate the protective effect of α-diammonium glycyrrhizinate phosphatidylcholine complex(DGLL) on chemical liver injury and its possible mechanisms.METHODS: The acute hepatic injury model inmice was induced by D-GalN.We measured the changes of activity of ALT and AST in serum and MDA,XOD, GSH-PX, NO, NOS and iNOS content of liver tissue.The model of chemical liver injury in mice was prepared.In vitro, primary cultured mice hepatocyte was injured by CCl4 , and the level of ALT in medium was measured to study protective effect of DGLL.RESULTS: DGLL could decrease the activities of ALT and AST in serum and the MDA, XOD, NOS and iNOS content of liver tissue, and was able to increase the activities of GSH-PX content of liver tissue.In vitro, it is indicated that GPLL had a notable protective effects on the hepatocyte injured by CCl4 and could suppress the level of ALT.CONCLUSION: DGLL had notable protective effect against acute liver injury in mice.

Key words: α-diammonium glycyrrhizinate phosphatidylcholine complex , chemical liver injury, primary hepatocyte