Abstract: AIM: To study the effects of proglucagonlike peptide-1, a long-acting prodrug glucagon-like peptide-1, on regulations of blood glucose levels and insulin secretion in non-diabetic mice. METHODS: Pro-GLP-1 was administered via scroute.Blood glucose levels were measured using a blood glucose meter.Plasma insulin concentrations were determined by ELISA. RESULTS: In C57BL 6 mice, native GLP-1 and Pro-GLP-1 decreased blood glucose, but Pro-GLP-1 had a more evident action.A single injection of Pro-GLP-1 dose-dependently reduced higher glucose following glucose load at least 3 h,and it had no effect on normal blood glucose.Moreover, it dose-dependently stimulated insulin secretion and significantly improved glucose tolerance after glucose challenge. CONCLUSION: These results demonstrate that Pro-GLP-1 facilitates a dose-dependently significant and prolonged glucose-lowering effect.

Key words: diabetes, glucagon-like peptide-1, prodrug, blood glucose, insulin