Leptin level change during acute myocardial infarction in rats

Abstract

Abstract: AIM: Experiments were carried out to explore the variation of leptin level during acute myocardial infarction of SD rats and the inter-relationship between leptin change and AMI progress.METHODS: Sixty Sprague-Dawlaw rats were randomized into 2 groups, 40 rats as the AMI group and 20 rats as the sham-operation group.AMIwas experimentally established by ligating/Left coronary artery anterior descending branch.Leptin level in serum and myocardium was measured at 1st, 2nd, 3rd and 4th week after ligation, respectively.Further, myocardial function and death area, as well as the left ventricle weight index were detected.RESULTS: The leptin level of the AMI group varied during the AMI process, experiencing a period with increasing/Leptin level at first and then a period with decreasing/Leptin level.The leptin level of the AMI group is significantly higher than that of the sham-operation group.The myocardial death area for the AMI group did not correlate with serum and myocardial leptin concentrations with a P value>0.05, while the left ventricle weight index correlated with both leptin concentrations in the early phase of AMI with P <0.05. Leptin concentration correlated well with SBP, DBP, LVESP, and +dp/dt_max, with P <0.05, while the correlations of leptin concentration with both LVEDP and-dpdt/_max were not significant, with P>0.05.CONCLUSION: Leptin is involved in the progress of AMI in the experimental SD rats.An increase in leptin level during AMI may signify myocardial deterioration.

Key words: acute myocardial infarction, leptin, SDrat

CLC Number:

R965.1

XU Tong-tong, YANG Yue-zhong. Leptin level change during acute myocardial infarction in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(2): 191-194.

References

[1] Meisel SR, EllisM, Pariente C, et al. Serum leptin levels increase following acute myocardial infarction[J]. Cardiology, 2001;95:206-211.
[2] Barouch LA, Berkowitz DE, Harrison RW, et al. Disruption of leptin signaling contributes to cardiac hypertrophy independently of body weight in mice[J]. Circulation, 2003;108:754-759.
[3] Shamsuzzaman AS, Winnicki M, Wolk R, et al. Independent association between plasma leptin and C-reactive protein in healthy humans[J]. Circulation, 2004;109:2181-2185.
[4] Zhang YY, Proenca R, Maffei M.Positional cloning of the mouse obese gene and its human homologue[J]. Nature, 1994;372:294-298.
[5] Mkata M, Yada T, Soejima N.Ieptin promotes aggregation of human platelets via the long form of its receptor[J]. Diabetes, 1999;48:426-429.
[6] Nhryama I, Nakata M, Yamam K.Effect of leptin on platelet and endothelialcells;obesity and arterial thrombosis[J]. Ann N Y Acad Sci, 2000;902:315-319.
[7] Konstantinide S, Schafer K, Koschnick S.Leptin-dependent platelet aggregation and arterial thrombosis suggests a mechanism for atherothrombotic disease in obesity[J]. J Gin Invest, 2001;108:1533-1540.
[8] Fishbein MC, Maclean D, Maroko PR.Experimental myocardial infarction in the rat[J]. Am J Pathol, 1978;90:57-70.
[9] Milavetz JJ, Raya TE, Johnson CS, et al. Survival after myocardial infarction in rats:captopril versus losartan[J]. J Am Coll Cardiol, 1996;27:714-719.
[10] Passsaro A, Calzoni F, Zamboni PF.Role of diabetes in influencing leptin concrenatration in eldly over 2 weight patients[J]. Eur J Endocrinol, 2001;145:173-179.
[11] 张政祥, 李红辉, 陈家林, 等.血清瘦素与原发性高血压和体脂及血脂的关系研究[J]. 高血压杂志, 2002;10:110-113.
[12] Stejskal D, Duzicka V, Bartek J.Leptinmia in persons withacute myocardial infarction[J]. Vntirk, 1998;44:588-592.
[13] Nkisel SR, Ellis M.Serum leptin levels increase following acute myocardial infarction[J]. Cardiology, 2001;95:206-211.
[14] Fujimaki S, Kanda T, Fujita K, et al. The significance of measuring plasma leptin in acute myocardial infarction[J]. J Int Nkd Res, 2001;29:108-113.
[15] Chu NF, Spiegelman D, Botamisligil GS, et al. Plasma insulin, leptin, and soluble TNF receptors levels in relation to obesity-related atherogenic and thrombogenic- cardiovascular disease risk factors among men[J]. Atherosclerosis, 2001;157:495-503.
[16] Christopher J, Ronald E, Willa AH.Adiposity, inflammation atherogenesis[J]. Endocrinology, 2003;144:2195-2200.
[17] Bouloumie A, Nbrumo T, LafontanM.Leptin induces oxidative stress in human endothelial cells[J]. FASEB J, 1999;13:1231-1238.
[18] Yamagishi SI, Eselstein D, Du XL, et al. Leptin induces mitochondrial superoxide production and monocyte chemoattractant protein-1 expression in aortic endothelial cells increasing fatty acid oxidation via protein kinase[J]. J Biol Chem, 2001;276:25096-25100.
[19] Queheberger P, Exner M, Sunder PR.Leptin induces endothelin-1 in endothelial cells in vitro[J]. Circ Res, 2002;90:711-718.
[20] 林季, 颜光涛, 郝秀华, 等.Leptin 及Orexin-ARI 在大鼠肠缺血-再灌注损伤中的应用[J]. 放射免疫学杂志, 2004;17:329-332.