Abstract: AIM: To extract and identify the soluble peptidoglycan from staphylococcus aureus.METHODS: The soluble peptidoglycan from staphylococcus aureus (ATCC25923) was released by short dose penicillin. Then, the soluble peptidoglycan was purified by sephadex G-100.The SLP reagent set was employed to identify the purified soluble peptidoglycan, and the production stimulated by the purified soluble peptidoglycan in RAW264.7 cell was observed by ELISA.RESULTS: Detected by the SLP reagent, the purified soluble peptidoglycan showed positive.The purified soluble peptidoglycan was capable of stimulating the production of TNF-αin RAW264.7 cell, and the amounts of TNF-αreleased were directly proportional to the concentrations of PGN.CONCLUSION: The soluble peptidoglycan from staphylococcus aureus can be obtained by penicillin and sephadex G-100, which has higher biological reactivities.

Key words: peptidoglycan, staphylococcus aureus, isolation, purification