Effects of atorvastatin on the proliferation and collagen synthesis of cardiac fibroblasts induced by endothelin-1

Abstract

Abstract: AIM: To investigate the effects of atorvastatin on the proliferation and collagen synthesis of cardiac fibroblasts (CFs) induced by endothelin-1 (ET-1). METHODS: Neonatal rat cardiac fibroblasts were randomly divided into 4 groups :control, ET-1, Atorvastatin, ET-1+atorvastatin (10^-7-10^-4 mol/L).CFs number was measured by MTT assay.Cell cycle distribution was determined with flow cytometer (FCM).[³H]-Proline uptake was evaluated by scinillation counting.Nitric Oxide (NO) was measured by chromatometry.RESULTS: 10^-6 mol/L ET-1 significantly increased the A490 value and [³H]-Pro incorporation, and decreased the secretion of NO compared with the control group (P<0.01).10^-7-10^-4 mol/L atorvastatin inhibited the above effects of ET-1 on CFs in a concentration-dependent manner (P<0.01 vs ET-1).In the ET-1 group, Phase S cell percentage was higher than that of contol, which was inhibited by atorvastatin (P<0.01 vs ET-1 and control).CONCLUSION: Atorvastatin dose-dependently inhibits the proliferation and collagen synthesis of cardiac fibroblasts induced by endothelin-1, which may be partially mediated by NO.

Key words: atorvastatin, cardiac fibroblasts, Endothelin-1, myocardial fibrosis

CLC Number:

R965.2

WANG Ai-li, CHENG Ling-ling, HU Jian-feng, CHENG Xin-yao. Effects of atorvastatin on the proliferation and collagen synthesis of cardiac fibroblasts induced by endothelin-1[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(9): 1058-1061.

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