Effects of tanshinone ⅡA on the expression of glutathione-s-transferaseμ2 in spontaneously hypertensive rats

Abstract

Abstract: AIM: To observe the effects of tanshinoneⅡA on the expression of glutathione-s-transferaseμ2 (GSTμ2) in spontaneously hypertensive rats, and to investigate the mechanism of the reversal of left ventricular hypertrophy by tanshinone ⅡA. METHODS: There are 3 groups in the experiment: control, spontaneously hypertensive rats(SHR), SHR treated by tanshinoneⅡA. At the endpoint of the experiment, hemodynamic parameters were measured. Then the rats were sacrificed, body weight (BW) and left ventricular weight (LVW) were measured, and the LVW BW ratios were calculated. The oxidative fluorescence dye dihydroethidium was determined by laser confocal fluorescent microscopy to evaluate in situ O2^-generation in the left ventricles. The expression of GSTμ2 was investigated by western blotting. RESULTS: Compared with control, the systolic blood pressure and LVW to BW ratios of SHR elevated markedly, the left ventricular function was decreased. The left ventricular hypertrophy was significantly reversed by tanshinone ⅡA treatment, but tanshinone ⅡA didn't decrease the systolic blood pressure of SHR. O2^-generation increased in SHR significantly, and it was decreased by tanshinone Ⅱ A. The expression and activities of GSTμ2 in left ventricular significantly decreased in SHR, however that was reversed by tanshinone ⅡA. CONCLUSION: Tanshinone ⅡA can significantly reverse the left ventricular hypertrophy in SHR, that might be associated with up-regulation of GSTμ2, decrease of O2^-and elimination of oxidative stress.

Key words: tanshinone ⅡA, left ventricular hypertrophy, spontaneously hypertensive rats, glutathione-s-transferase μ2, oxidative stress

CLC Number:

R965.2

ZHOU Si-gui, WANE Ping, WANG Zhao-he, PAN Xue-diao, LIU Pei-qing. Effects of tanshinone ⅡA on the expression of glutathione-s-transferaseμ2 in spontaneously hypertensive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(1): 57-61.

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