Effect of estrogen on the monoamine neurotransmitter in the serum of rats with post-stroke depression

Abstract

Abstract: AIM: To study the effect of estrogen on the monoamine neurotransmitter in the serum of rats with post-stroke depression(PSD).METHODS: The female Sprague Dawley rats weighted 220-230 g were randomly divided into control group, stroke group, PSD group, antidepressant treatment group and estrogen treatment group according to the scores of Open-Field test.Rats in all groups were performed ovariectomy first.Rats in control group were feed commonly. Seven days later, rats in the stroke group, PSD group, drug treatment group and estrogen treatment group were all duplicated into focal ischemia by means of middle cerebral artery occlusion, then the rats in PSD group, drug treatment group and estrogen treatment group received isolated feed and restraint stress to duplicate PSD model.Rats in drug treatment group were received Paroxetine and in rats estrogen treatment group were received the E2-containing capsule that were embed in subcutaneous by operation.The E2-containing capsules produced circulating concentrations of E2 within the range observed during the diestrous phase of the estrous cycle.After that, the behavioral evaluations were done with Open-Field test, these rats were sacrificed and their blood were taken.The mononeurotransmitters included NE and 5-HT in serum were detected by HPLCECD.RESULTS: The spontaneous behavior and the concentration of NE and 5-HT in serum of the PSD rats decreased significantly in comparison with those in control group(P <0.05), the spontaneous behavior and the concentration of NE and 5-HT in serum of the rats in estrogen treatment group increased significantly in comparison with those in PSD group(P <0.05), the spontaneous behavior and the concentration of 5-HT in serum of the rats in estrogen treatment group increased significantly as those in drug treatment group (P > 0.05), the concentration of NE in estrogen treatment group increased non-significantly in comparison with that in drug treatment group(P <0.05).CONCLUSION: The spontaneous behavior could be improved and the 5-HT was increased by estrogen in PSD rats. That may protect the brain of PSD rats.

Key words: estrogen, post-stroke, depression, norepinephrine, 5-hydroxytryptamine, HPLC-ECD

CLC Number:

R965.2

WANG Hong, SHAO Bei, Kevin O` Byrne, LI Xiao-feng, LIN Yuan-shao, YANG Jian-rong, WANG Zhi-qiang, FAN Hong. Effect of estrogen on the monoamine neurotransmitter in the serum of rats with post-stroke depression[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(12): 1375-1380.

References

[1] Longa EZ, Weinstein PR, Carlson, et al.Reversible middle cerebral artery occulusion without craniectomy in rats [J].Stroke, 1989, 20(1):84-91.
[2] Bederson JB, Pitts LH, Tsuji M, et al.Rat middle cerebral artery occlusion:evaluation of the model and development of a neurologic examination[J].Stroke, 1986, 17(3):472-476.
[3] 倪贵华, 邵蓓, 范虹.卒中后抑郁实验动物模型的研究[J].温州医学院学报, 2006, 36(6):524-526.
[4] Li XF, Kinsey-Jones JS, Knox AM, et al.Neonatal lipopolysaccharide exposure exacerbates stress-induced suppression of luteinizing hormone pulse frequency in adulthood[J].Endocrinology, 2007, 148(12):5984-5990.
[5] Gronli J, Murison R, Bjorvatn B, et al.Chronic mild stress affects sucrose intake and sleep in rats[J].Behav Brain Res, 2004, 150(1/2):139-147.
[6] Gronli J, Murison R, Fiske E, et al.Effects of chronic mild stress on sexual behavior, locomotor and consumption of sucrose and saccharione solutions[J].Physiol Behav, 2005, 84(4):571-557.
[7] Blokland A, Lieben C, Deutz NE.Anxiogenic and depressivelike effects, but no cognitive deficits, after repeated moderate tryptophan depletion in the rat[J].J Psy chopharmacol, 2002, 16(1):39-49.
[8] Kugaya A, Epperson CN, Zoghbi S, et al.Increase in prefrontal cortex serotonin 2A receptors following estrogen treatment in postmenopausal women[J].Am J Psychiatry, 2003, 160(8):1522-1524.
[9] Alonso de Lecinana M, Egido JA.Estrogens as neuroprotectants against ischemic stroke [J].Cerebrovasc Dis, 2006, 21(2):48-53.
[10] Kochanowicz J, Krejza J, Koroza O, et al.Influence of estrogens on the impedance of cerebral blood vessels[J]. Neurol Neurochir Pol, 2005, 39(3):175-180.
[11] Tanaka R, Yamashiro K, Mochizuki H, et al.Neurogenesis after transient global ischemia in the adult hippocampus visualized by improved retroviral vector[J].Stroke, 2004, 35(6):1454-1459.
[12] Saravia F, Revsin Y, Lux-Lantos V, et al.O estradiol restores cdll proliferation in dentate gyrus and subventricular zone of streptozotocindiabetic mice[J].J Neuroendocrinol, 2004, 16(8):704-710.
[13] Murashov AK, Pak ES, Hendricks WA, et al.17beta-Estradiol enhances neuronal differentiation of mouse embryonic stem cells[J].FEBS Ltee, 2004, 569(1/3):165-168.
[14] Wei L, Ying DJ, Cui L, et al.Necrosis, apoptosis and hybrid death in the cortex and thalamus after barrel cortex ischemia in rats[J].Brain Res, 2004, 1022(1/2):54-61.
[15] Sortino MA, Chisari M, Merlo S, et al.Glia mediates the neuroprotective action of estradiol on beta-amyloid-induced neuronal death[J].Endocrinology, 2004, 145(11):5080-5086.
[16] Liu X, Fan XL, Zhao Y, et al.Estrogen provides neuroprotection against activated microglia-induced dopaminergic neuronal injury through both estrogen receptor-alpha and estrogen receptor-beta in microglia[J].J Neurosci Res, 2005, 81(5):653-665.
[17] 肖红, 姚辉, 侯刚, 等.抑郁症患者接受治疗前后血浆单胺类神经递质代谢产物的差异[J].中国临床康复, 2005, 9(8):246.
[18] Hirata K, Tanaka H, Zeng XH, et al.The role of the basal ganglia and cerebellum in cognitive impaiment a study using event-related potentials[J].Suppl Clin Neurophysiol, 2006, 59:49-55.