Effects of tripterine on the immunosuppression action and expression of IL-6 mRNA in mice

Abstract

Abstract: AIM:To investigate the effects of tripterine on the immunosuppression action and the over expression of IL-6 gene in mice.METHODS:The carbon particle clearance reaction test, delayed hyper-sensitivity reaction test, serum hemolysin assay and lymphocyte transfer test were applied to observe the immunosuppression action of tripterine.Mice were orally pretreated with tripterine, and the IL-6 mRNA level was determined by RT-PCR.RESULTS:The level of serum hemolsin in mice treated with triperine (400 μg/kg) was much lower than that of control group, suggesting that tripterine may suppress the level of serum hemolsin in mice in dose dependent manner.Tripterine (400 μg/kg) had an obvious effect of immunosuppression on mice cells by relieving the delayed hypersensitivity reaction, and tripterine (0.25 μg/mL) had inhibitory effects on the activation and proliferation of mice T lymphocytes stimulated by PHA.In addition, significant increase of IL-6 mRNA level induced by CCl₄ was remarkably decreased by 800 μg/kg tripterine.CONCLUSION:Tripterine suppresses the immunological function of mice to some extent and inhibits the over expression of IL-6 gene in mice liver which is related to proinflammatory cytokines.

Key words: tripterine, immunosuppression, IL-6, expression of mRNA

CLC Number:

R963
R285.5

LI Meng-qiu, DOU Jie, DU Wei, LI Xin-ya, LIN Jian, ZHOU Chang-lin. Effects of tripterine on the immunosuppression action and expression of IL-6 mRNA in mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(2): 158-163.

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