Abstract: AIM:To study the influence of triptolide on the angiotension Ⅱ(Ang Ⅱ)-induced neonatalrat myocardial fibroblasts (CFb) proliferation and pro α₁(Ⅰ) collagen synthesis, and to explore the mecha-nism triptolide on myocardial fibrosis. METHODS: Neonatal rat myocardial fibroblasts were treated with 1×10^-7 mmol/L angiotensin Ⅱ (Ang Ⅱ, 1 ×10^-7mmol/L) to make fibrosis model. The inhibition of triptolide on CFb proliferation was evaluated by MTT assay, the synthesis of pro α₁(Ⅰ) collagen was observed by RT-PCR and Western blotting. RESULTS: In a concentration range, triptolide (0. 5, 1. 0, 2. 0 and 4. 0 μg/mL) significantly inhibited the Ang Ⅱ-induced CFb proliferation and pro α₁(Ⅰ) collagen synthsis in a dose-dependent way. CONCLUSION: Triptolide might attenuate myocardial fibrosis induced by angiotensin Ⅱ through inhibiting CFb proliferation and pro α₁(Ⅰ) collagen synthsis.

Key words: triptolide, myocardial fibroblast, angiotensin Ⅱ, pro α₁(Ⅰ) collagen