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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2008, Vol. 13 ›› Issue (6): 610-613.

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Nordosin enhanced inhibition of FK506 on the secretion of IL-2 by lymphocyte derived from spleen of mice in vitro

DU Jun-ming1, WU Ping1, SUN Li-juan2, LIU Jian-wen2, LI Ji-yu1, QUAN Zhi-wei1   

  1. 1Department of General Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 20092, China;
    2State Key Laboratory of Bioreactor Engineering & School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
  • Received:2007-11-05 Revised:2008-06-11 Published:2020-10-14

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Abstract: AIM: To investigate the effect of Nordosin in inhibiting proinflammatory cytokine with FK506 in vitro and potential applied value in the transplantation immunity.METHODS: ConA-stimulated T-cell proliferation model was made.Then, the expression of IL-2 both in protein and mRNA lever were investigated by ELISA and RT-PCR.Furthermore, the expression of IL-2 receptor, CD25+ on surface of T lymphocyte was also measured by FCM.RESULTS: Compare with those by FK506 use along, Nordosin enhanced the inhibition of FK506 on the expression of IL-2 significantly (P<0.01).The expression of IL-2 mRNA and its receptor CD25+ were also inhibited by their combining medication (P<0.01).CONCLUSION: Nordosin could enhance the inhibition of FK506 on IL-2 secretion significantly, Nordosin maybe become an immunodepression assistant medication to enlarge the pharmacological effect of FK506.

Key words: Nordosin, FK506, CD25+, IL-2, transplantation immunity

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