Oxidative lesions induced by medical SiO2 nanoparticles on normal human lung cells

Abstract

Abstract: AIM: To investigate the medical SiO₂ nanoparticles induced cytotoxicity and its mechanism on normal human lung cells (MRC-5 cells) in vitro. METHODS: The MRC-5 cells were exposed with different concentrations of SiO₂ nanoparticles for 48 h. The cell survival rate was tested by MTT assay and the cell morphological changes were observed with HE staining.The activities of reactive oxygen species (ROS), glutathione (GSH) and superoxide dismutase (SOD) were determined to evaluate the oxidative damage after SiO₂ exposure. RESULTS: Exposure with high concentrations above 0.4 mg/mL and 1.0 mg/mL of 21.6 nm and 48.6 nm SiO₂ nanoparticles decreased the cell survival rate in a dose-dependent manner.The median inhibitory concentrations (IC50) were 0.8 mg/mL and 1.9 mg/mL respectively.Morphological observation revealed cell shrinkage and nuclear condensation after exposure.The reduced GSH content as well as SOD activity were decreased in SiO₂ nanoparticles exposed cells than that of control. CONCLUSION: Medical SiO₂ nanoparticles exposure resultes in a dosedependent cytotoxicity in cultured MRC-5 cells that was associated with increased oxidative stress.

Key words: medical SiO₂ nanoparticles, cytotoxicity, oxidative stress

CLC Number:

R965.2

LI Ai-si, HUANG Yong-ping, LIU Jian-wen, LAN Min-bo, GAO Feng, XU Hao-liang, CHEN Ming-cang, XU Yi-chun. Oxidative lesions induced by medical SiO₂ nanoparticles on normal human lung cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(10): 1115-1120.

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Oxidative lesions induced by medical SiO₂ nanoparticles on normal human lung cells

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