Effects of dexamethasone on toll-like receptor 4 mediated signaling pathways in mice dendritic cells

Abstract

Abstract: AIM: To observe the effects of dexamethasone on the expression of toll-like receptor 4 (TLR4) and the activation of NF-κB of dendritic cells (DC), and investigate the mechanism of dexamethasone suppressing differentiation, maturation, and function of DC. METHODS: DC generated from C57BL/6 murine bone marrow cells were induced by GM-CSF and IL-4.Dexamethasone was added in the DC's culture system, the cells were analyzed by flow cytometry (FCM) to determine the expression of TLR4 &CD11c, and by electrophoretic mobility shift assay (EMSA) to verify the activation of transcription factor NF-κB of DC. RESULTS: Dexamethasone could suppress the expression of CD11c and the activation of NF-κB with a dose-effect relationship in DC, but it could not suppress the expression of TLR4 of DC. CONCLUSION: The mechanism of dexamethasone involved in inhibition of differentiation, maturation, and function of DC is perhaps related to suppression of NF-κB activation.

Key words: dendritic cells, dexamethasone, tolllike receptor 4, nuclear factor kappa B

CLC Number:

R392

WU Xiang-ling, HE Xiao-kui, LI Bo, HU Yong-xiu. Effects of dexamethasone on toll-like receptor 4 mediated signaling pathways in mice dendritic cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(9): 1023-1027.

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