Abstract: AIM: To study the transmigration ingredient of blood serum of Sinisan Freeze-dry Powder in order to determine that Sinisan enhances the sleep function with drug effect material base. METHODS: The HPLC method was used to do gradient elution, and the transmig ration ingredient of blood serum of Sinisan Freezedry Pow der was determined.The chromatograph condition: Hypersil C18 column (4.6 mm×250 mm, 5 μm), speed of flow 1.0 mL/min, column warm 30 ℃, flowing A is acetonit rile ;flowing B is 0.05 % phosphorus acid water, the flow ingA gradient elution (10 %-90 % aceto nitrile), the analysis time is 150 min.RESULTS: 14 kinds of transmig rationing redient sappeared in blood serum after seven days successive medication with Sinisan Freeze-dry Powder.CONCLUSION: The specificity and characteristic of the blood serum HPLC fingerprint mark in Sinisan are good, which can be used in the transmig ration ingredient of blood serum of Sinisan Freeze-dry Powder.

Key words: Sinisan Freeze-dry Powder, Transmigration ingredient of blood serum, Fingerprinting