Abstract: AIM: To investigate the effect of immune regulation of I L-7 gene transfected into human carcinoma line Tca8113. METHODS pBK-CMV/IL-7 plasmid was transfected into human tongue squamous carcinoma cell lines. The effect of the supernatant of carcinoma culture on the proliferation of lymphocytes was measured using MTT method.The concentrations of TGF-β1, VEGF and IL-10 were determined by ELISA.Human carcinoma cells lines with pPK-CMV/I L-7 were injected into peritoneal of mice, and the tumor formed in peritoneal was detected.The killing activity of NK was analyzed by MT T. RESULTS:Compared with control group, the supernatant from carcinoma cells culture could significantly suppress the proliferation of lymphocytes (P<0.05).The supernatant levels of TGF-β1, VEGF and IL-10 were obviously decreased.The tumor volume in experimental group were fewer then those in control group(P<0.05).The killing activity of NK was significantly increased (P<0.05). CONCLUSION: IL-7 gene transfection into human carcinoma line Tca8113 could inhibit tumor growth by reducing secretion of immune suppressive factors, enhancing lymphocyte proliferation and increasing the killing activity of NK.

Key words: IL-7, Immune regulation, Human tongue squamous carcinoma cell lines