Effects of sodium hydrosulfide on apoptosis in lung injury induced by ischemic and reperfusion in rats

Abstract

Abstract: AIM: To investigate the effect of sodium hydrosulfide (NaHS), the donor of H₂S, on apoptosis during lung ischemia and reperfusion injury (LIRI) in rats. METHODS: Single lung ischemia and reperfusion animal model were used in vivo. A total of 30 Sprague-Dawley(SD) rats were divided at random into 3 groups as follows: sham operation control (Sham) group, lung ischemia-reperfusion(LIR) group, and LIR-sodium hydrosulfide(LIR+NaHS) group. Each group consisted of 10 rats. Under anesthetized, all rats were underwented the procedure of operation as the following: opening the left chest and isolating the hilus of lung, through which a suture was passed. The Sham group didn't endure the pulmonary hila's ligation while the LIR group was subjected to a 30-minute ischemia and 120-minute reperfusion insult. The lung tissues sampled at the end of experiments were assayed for lung/body weight ratio (LW/BW).The tissue slides were also stained by immunohistochemistry (IHC) for bcl-2/bax to detect the expression of bcl-2/bax protein in lung and to analyze the optical density. The cell apoptosis in lung tissue were determined by TUNEL. The histological and ultrastructure changes were observed by light microscope and electron microscope. The injured alveoli rate (IAR) was counted as well. RESULTS: As compared with LIR group, in intima and extima of small pulmonary artery, alveoli, and bronchiole epithelia, the expression of bcl-2 proteins and the ratio of bcl-2/bax were increased, and the expression of bax protein was decreased in LIR+NaHS group. The values of AI, LW/BW and IAR showed significantly lower than that in LIR group (P<0.05). Meanwhile, abnormal changes of the lung tissue in morphologically were markedly lesseed in LIR-NaHS group. CONCLUSION: NaHS produces a notable protective effect on LIRI in rats by up-regulating bcl-2 protein expression, down-regulating bax protein expression in lung tissue and regulating the balance of bcl-2 and bax to decrease apoptosis.

Key words: Ischemia-reperfusion injury, Lung, Apoptosis, Sodium hydrosulfide

CLC Number:

R965.2

WANG Xing-min, JI Hai-feng, WANG Wan-tie, SHI Lu, JIA Xu-guang. Effects of sodium hydrosulfide on apoptosis in lung injury induced by ischemic and reperfusion in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(5): 496-501.

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