Protective effect of MIF siRNA mediated by lipid microbubbles on acute lung injury in mice

Abstract

Abstract: AIM: To investigate the protective effect of MIF siRNA mediated by ultrasound lipid microbubbles gene transfer system and evaluate the efficacy of this gene transfer system.METHODS: The model of acute lung injury was induced by LPS in mice which were randomly divided into 4 groups: normal control group (Con), LPS stimulation (LPS), LPS + PC + MIF siRNA treatment group (PC + MIF siRNA), LPS + WP + MIF siRNA treatment group (WP + MIF siRNA). The MIF siRNA was transfected by microbubbles ultrasound. These methods including EMSA, Western-Blot, ELISA and HE stained for histopathological examination were used to observe the expressions of NF-κB and IκB-α, to detect the levels of inflammatory mediators of TNF-α, IL-1β, IL-6, and to assess the pathological changes.RESULTS: MIF siRNA was carried and transferred by WP + MIF siRNA which up-regulated the expression of IκB-α in cytoplasm and significantly inhibited the expression of NF-κB in nucleus, then inhibited the levels of inflammatory mediators of TNF-α, IL-1β, IL -6 and improved the pathological changes. But the PC+ MIF siRNA treatment group has no any improvement on acute lung injury in mice.CONCLUSION: MIF siRNA can be effectively transferred by lipid microbubbles gene transfer system and have protective effect on acute lung injury in mice.

Key words: Lipid microbubbles, MIF siRNA, Acute lung injury

CLC Number:

R965

JIANG Yong-nan, MO Hong-ying, CHEN Jian-hai. Protective effect of MIF siRNA mediated by lipid microbubbles on acute lung injury in mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(7): 737-741.

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