Improvement effect of salvianolate on small intestinal mucosal barrier in cirrhotic rats

Abstract

Abstract: AIM: To investigate the effect of salvianolate on small intestinal mucosal barrier in rats with CCl₄-induced liver cirrhosis.METHODS: Cirrhosis was induced in rats using CCl₄ (0.3 mL/kg ). Rats were divided randomly into four groups:untreatmeat, low-dose salvianolate(12 mg/kg), medium-dose salvianolate(24 mg/kg), and high-dose salvianolate(48 mg/kg). They were all treated for 2 weeks.Another 10 healthy rats were enrolled as normal control. Mortality of cirrhotic rats in each group were evaluated after treatment. Serum samples were collected from the portal vein for the detection of endotoxin. Tissue samples from the ileocecum were observed under light microscopy for morphological changes.A contrasting study on the expression of secretary immunoglobulin A(sIgA) were performed in each group by immunohistochemical staining method.RESULTS: The mortality of cirrhotic rats in the untreatmeat group was 42.86%. No cirrhotic rat died in the high-dose salvianolate group. The serum endotoxin level was highter in the untreatmeat group than that in salvianolate group(P<0.05). Intestinal mucosal and villous atrophy were observed in the untreatmeat group, with necrosis and shedding of the intestinal mucosal epithelium. These pathological changes were reversed in the salvianolate-treated groups.The expression of sIgA in intestinal mucosa were reduced obviously in the untreatmeat group, in compared with those in the salvianolate-treated groups and healthy rats group. The integral optical density of immunohistochemical staining image in untreatmeat group was obviously lower than that in high-dose salvianolate-treated group (P<0.01). CONCLUSION: Salvianolate is helpful for reducing endotoxin level,ameliorating injury of intestinal mucosa,enhancing immune function of intestinal mucosa in rats with CCl₄ induced cirrhosis,and therefore improving the barrier function of intestinal mucosa.

Key words: Salvianolate, Cirrhosis, Endotoxin, Intestinal mucosa, sIgA

CLC Number:

R965.2

YANG Dan-hong, YE Zai-yuan, HE Xu-jun, ZHANG Qin, ZHOU Wei-ming, XU Wen-juan, LV Huo-xiang. Improvement effect of salvianolate on small intestinal mucosal barrier in cirrhotic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(7): 758-763.

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