Nux vomica inhibiting apoptosis of spinal cord injury rabbit

Abstract

Abstract: AIM: To explore whether nux vomica treats spinal cord injury by inhibiting apoptosis.METHODS: The animal model of SCI was made in rabbits by modified Allen's method. 60 rabbits were randomly divided into 4 groups: the sham group, control group, 40 mg nux vomica group (group M40) and 80 mg nux vomica group (group M80). After treating for 1, 7 and 14 days , the NO level in the spinal cord was detected with the Griess reaction and the expressions of c-fos protein, tested Bax and Bcl-2 protein were examined by Western blot method (Western blotting).RESULTS: Compared with the control group, the expressions of NO, c-fos protein, Bax protein were decreased, while the expression of Bcl-2 protein was increased after treating for 7 days and 14 days in the group M40 and group M80. After 7 days' treatment, the NO contents in the group M40 and group M80 were lower than the control group (P＜0.05). In the group M80, the expressions of c-fos protein were lower than the control group after 7 days' treatment (P＜0.05) and 14 days' treatment (P＜0.01); the expressions of Bax protein were lower than the control group after 7 days' treatment (P＜0.05) and 14 days' treatment (P＜0.05); the expression of Bcl-2 protein was higher than the control group after 7 days' treatment (P＜0.05); the ratio of Bax/Bcl-2 was lower than the control group after 7 days' treatment (P＜0.05).CONCLUSION: Nux vomica can hold back the secondary spinal cord injury and accelerate the spinal nerve function by reducing the expressions of NO and c-fos protein, and cutting the ratio of Bax/Bcl-2 to suppresse the cell apoptosis.

Key words: Nux vomica, Spinal cord injury, Apoptosis

CLC Number:

R965.2

ZHOU Hong-ying, HOU Qun, QI Guan-shu, QIU Chang-lin. Nux vomica inhibiting apoptosis of spinal cord injury rabbit[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(8): 880-885.

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