Effects of Notoginsenoside monomer R1 on the expression of ERK1/2 in PASMCs

Abstract

Abstract: AIM: To study the effect of monomer R₁ aleviating HHPV via MAPKs signal pathway.METHODS: To culture pulmonary arterial smooth muscle cells (PASMCs) of male SD rats primarily, PASMCs were randomly divided into 6 groups: control group(N), hypoxia hypercapnia group(H), DMSO incubation group (H_D), R₁ groups(R₈, R₄₀, R₁₀₀). Incubated cells were collected. p-Erk1/2 was investigated through western blot, and ERK1 mRNA and ERK2 mRNA were detected via RT-PCR.RESULTS: The expretion of p-ERK protein was weak in the N group, and in R₈,R₄₀ and R₁₀₀ groups the level of p-ERK was reduced significantly(P＜0.01), declining mostly in R₈ group. The expretion of ERK1 mRNA and ERK2 mRNA was not obvious in N group, and comparing with H and H_D groups it markedly down-regulated in R₈, R₄₀ and R₁₀₀ groups(P＜0.01, P＜0.05), reaching its lowest point in R₈ group.CONCLUSION: ERK1/2 signal pathway may play an important role in HHPV in rat.The effect of R₁ on relaxing HHPV may be related to inhibiting expression of ERK1/2.

Key words: Hypoxic hypercapnia, Pulmonary arterial hypertension, ERK1/2 signal pathway, Notoginsenoside monomer R₁

CLC Number:

R965.2

TANG Lan-lan, WANG Shu-jun, LI Guan-long, WANG Yuan-yuan, ZHOU Jun-hui, ZHAO Shan, LIU Ya-kun, WANG Wan-tie. Effects of Notoginsenoside monomer R₁ on the expression of ERK1/2 in PASMCs[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(11): 1216-1221.

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Effects of Notoginsenoside monomer R₁ on the expression of ERK1/2 in PASMCs

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