Effect of CGRP on the CDK2 and Cyclin E of rat vascular smooth muscle cell

Abstract

Abstract: AIM: To study the effect of calcitonin gene-related peptide (CGRP) on cyclin-dependent kinase 2(CDK2) and Cyclin E of vascular smooth muscle cell (VSMC), and to elute the mechanism of inhibitory effect of CGRP on proliferation of VSMC in cell cycles. METHODS: VSMC were prepared from thoracic aortas of male Sprague-Dawley rat by explanting method. The cell viability and cell cycle were determined by MTT and Flow Cytometry, respectively. Western Blot was used to observe expressions of CDK2 and Cyclin E of VSMC. RESULTS: Pretreatment of VSMC with CGRP significantly inhibited cells viability, decreased the proportion of S phase and increased ratio of G₀/G₁ that were induced by 10% FBS, simultaneously, CGRP down-regulated the expressions of CDK2 and Cyclin E when cultured cells with 10% FBS at 12, 24 and 48 hours.CONCLUSION: CGRP could inhibit the cell cycle of VSMC G₀/G₁ phase to S phase transition, by which the mechanism maybe involved in the decrease expressions of CDK2 and Cyclin E.

Key words: CGRP, Vascular smooth muscle cell, Cell cycle, CDK2, Cyclin E

CLC Number:

R965.2

DENG Shui-xiu, ZENG Si-yu, REN Jun-fang, ZHENG Yuan-bin, LIAO Duan-fang, QIN Xu-ping. Effect of CGRP on the CDK2 and Cyclin E of rat vascular smooth muscle cell[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(3): 249-253.

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