Effects of magnesium lithospermate B on the expression of heme oxygenase-1 in HEK293T cells

Abstract

Abstract: AIM: To further confirm the effects of magnesium lithospermate B (LAB) on the expression of heme oxygenase-1 (HO-1) in HEK293T cells induced by high glucose.METHODS: The intracellular reactive oxygen species in cells was determined by flow cytometry .The expression levels of HO-1 mRNA and HO-1 protein in HEK293T cells treated with high glucose and LAB were determined by quantitative reverse transcription polymerase chain reaction (QRT-PCR) and western blotting.RESULTS: Flow cytometry showed that the ROS concentration was increased markedly in HEK293T cells induced by high glucose and H₂O₂ and LAB inhibited the ROS generation of HEK293T cells. The expression levels of HO-1 mRNA and protein were increased significantly in HEK293T cells treated with high glucose and reached the peak value at 24 h.Compared with high glucose group,the expression levels of HO-1 mRNA and protein were obviously enhanced after cells were treated with 10,50,100 mol/L LAB.CONCLUSION: LAB not only can directly clear up the excess ROS in cells under oxidative stress, but also it play a antioxidant role via an increase of downstream gene HO-1 expression.

Key words: Magnesium lithospermate B, Reactive oxygen, Heme oxygenase-1, HEK293T

CLC Number:

R965.2

REN Xian, HOU Rui-ying, QU Jian, SHEN Jie, ZHOU Hong-hao, LIU Zhao-qian. Effects of magnesium lithospermate B on the expression of heme oxygenase-1 in HEK293T cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(5): 481-487.

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