Protective effect of cistanchis glycosides on ethanol-induced damage in the primary hepatocytes of mice

Abstract

Abstract: AIM: To discuss the protection of cistanchis glycosides on ethanol-induced damage in the primary hepatocyte of mice.METHODS: The primary hepatocytes were collected by perfusion in situ method. The cell livability was evaluated by MTT, fluorescence microscopy and flow cytometry were used to evaluate the cell apoptosis and necrosis, immunohistochemistry was used to detect the expression of bcl-2 and c-fos.RESULTS: After the primary hepatocytes were damaged by alcohol,the cell livability decreased, apoptosis and necrosis were obvious,the expression of bcl-2 was decreased while that of c-fos was increased.Cistanchis glycosides could increase the cell livability, ameliorate apoptosis and necrosis, increase the expression of bcl-2, and decrease the expression of c-fos in a dose-dependent manner.CONCLUSION: The protective effects of cistanchis glycosides on ethanol-induced liver damage in the primary hepatocytes is associated with increasing the expression of bcl-2, and decreasing the expression of c-fos; resulting less apoptosis, less necrosis, and increasing the cell livability.

Key words: Cistanchis glycosides, Primary hepatocyte, Ethanol-induced liver damage, Apoptosis

CLC Number:

R965.2

LUO Hui-ying, LIU Yuan, YANG Huan. Protective effect of cistanchis glycosides on ethanol-induced damage in the primary hepatocytes of mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(8): 856-861.

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