Abstract: AIM: To investigate therapeutic time window of MC-002 on the focal cerebral ischemia-reperfusion rats.METHODS: The models with cerebral ischemicre-reperfusion were induced by intraluminal middle cerebral artery occlusion (MCAO) with a nylon monofilament suture, 2 h occlusion followed by 22 h reperfusion. Therapeutic medicine was intravenously administered after reperfusion for 0, 0.5, 1, 2, 4 h. The effect of MC-002 at different doses on neurological scores, infarct sizes, water contents, pathological changes and the biochemical indexes of CIRI rats were examined.RESULTS: MC-002 administered at 0, 0.5, 1, 2 h after reperfusion significantly improved the CIRI rats' neurological function and reduced the cerebral infarct size and edema; The content of MDA and LD in brain were markedly decreased (P<0.05 and P<0.01), and the activity of SOD was significantly enhanced (P<0.01). The pathological observations showed that neuronal cells shape and number were obviously improved after treated with MC-002 compared with model groups.CONCLUSION: The results showed that the therapeutic time window of MC-002 extended for up to 2 h after CIRI.

Key words: MC-002, Focal cerebral ischemiareperfusion, Therapeutic time window