Abstract: AIM: To investigate the cytotoxic effect and mechanism of ampelopsin sodium (AMP-Na) on human lung adenocarcionma cell line SPC-A-1 by alone or combined with carboplatin.METHODS: The inhibitory effects on proliferation of human lung cancer cell line SPC-A-1 were examined by MTT colorimetric assay. Flow cytometry (FCM) was employed to analyze the cell apoptosis.RESULTS: Experimental study on cytotoxicity in vitro showed that AMP-Na decreased the survival rate of SPC-A-1 cells in a concentration-dependent manner. There was a synergistic cytotoxic effect on SPC-A-1 cells treated with AMP-Na combined with carboplatin. The apoptosis rate in group treated with AMP-Na combined with carboplatin was significantly increased as compared with that in group treated with carboplatin alone.CONCLUSION: AMP-Na decreases SPC-A-1 cells survival rate in a concentration dependent manner, and shows significant cytotoxic activity. There is a synergistic cytotoxic effect on SPC-A-1 cells treated with AMP-Na combined with carboplatin. The mechanism of AMP-Na is probably that it can induce SPC-A-1 cells apoptosis.

Key words: Ampelopsin sodium, Carboplatin, Human lung adenocarcionma cell line SPC-A-1, Apoptosis