Effects of icariin on monoamine and amino acid neurotransmitters in senescence accelerated mouse prone/10

Abstract

Abstract: AIM: To investigate the effects of icariin(ICA) on monoamine and amino acid neurotransmitters in senescence accelerated mouse prone/10(SAMP10). METHODS: The 8-month-old SAMP10 were randomly divided into model group, Donepezil Hydrochloride(DON)-treated SAMP10 group (1 mg/kg) and ICA-treated SAMP10 groups(50, 100, 200 mg/kg), with twelve mice in each group. Twelve senescence accelerated mouse/resistance1(SAMR1) with the same age were used for normal control. After 30 days of oral administration, HPLC with electrochemical detection (EC) was used to measure norepinephrine, 5-hydroxytryptamine, dopamine and its metabolites 3,4-phenyl acetic acid and homovanillic acid, and glutamic acid,glutamine,aspartic acid, γ-aminobutyric acid,taurine,glycine.RESULTS: ICA could significantly reduce the levels of glutamic acid, glutamine and γ-aminobutyric acid in the cerebral cortex of SAMP10 (P<0.01), and increase the levels of norepinephrine, dopamine, 3, 4 - phenyl acetic acid,5 - hydroxytryptamine, homovanillic acid, aspartic acid and taurine (P<0.01 or P<0.05), but glycine had no significant effect(P>0.05). CONCLUSION: The improvement of ICA on the ability of learning and memory in SAMP10 may be related to elevating levels of monoamine neurotransmitter, regulating the balance of excitatory amino acids and regulating the balance of inhibitory amino acids.

Key words: Icariin, Senescence accelerated mouse prone/10, Monoamine, Amino acid

CLC Number:

R965.2

GAO Lin-na, HE Xiao-li, TANG Qian-qi, HE Guo-rong, BI Ming-gang. Effects of icariin on monoamine and amino acid neurotransmitters in senescence accelerated mouse prone/10[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(10): 1081-1086.

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