Effects of genistein on the growth of ovarian cancer cell SKOV3 by regulating miR-27a and target gene expression

Abstract

Abstract: AIM: To investigate the effects of genistein on the growth of ovarian cancer cell SKOV3 and potential mechanism. METHODS: The expression of the miR-27a in formalin-fixed paraffin-embedded (FFPE) ovarian cancer tissues and benign ovarian tissues were examined by RT-PCR. SKOV3 cells were treated with different concentration of genistein. Then the SRB assays were performed to assess the growth inhibition of genistein to ovarian cancer cells. RT-PCR and Western blot were used to test expression of miR-27a and Sprouty2 respectively. RESULTS: The expression levels of miR-27a in FFPE ovarian cancer tissues were significantly higher than those seen in benign ovarian tissues. Genistein could markedly decrease human ovarian cancer cell growth in time- and dose- dependent manner and downregulate the expression of miR-27a, which was accompanied by significantly increased expression of Sprouty2. CONCLUSION: Genistein has an inhibitory effect on the growth of ovarian cancer cells, which was due to the downregulation of miR-27a expression.

Key words: Genistein, Ovarian cancer, Growth, MiR-27a, Sprouty2

CLC Number:

R965.1

XU Lin-lin, SUN Qing-min, LUO Xuan, ZHAI Su-lan, LI Ping, ZHAO Xiao-mei, WANG Xue-rong. Effects of genistein on the growth of ovarian cancer cell SKOV3 by regulating miR-27a and target gene expression[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(12): 1321-1326.

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