The impact of propofol pretreatment on hypoxia/reoxygenation in cultured hippocampal neurons metallothionein-3 protein expression

Abstract

Abstract: AIM: To observe the effects of propofol on caltured hippocampal neurons hypoxia and reoxygenation damage response and metallothionein protein-3(metllothionein-3,MT-3) in which the role.METHODS: Hippocampal neuron cells were cultured for 7-10 days for use. Hypoxic conditions were attained 4 h and reoxygenation was achieved with normoxia conditions for another 24 h. The expression level of metallothionein-3 (MT-3) was detected by Western blot; By using MT-3 specific siRNA, MT-3 was silenced and the effect of propofol on cell survival rate was evaluated by MTT method .RESULTS:In cultured hippocampal neuron cells, pretreatment with different concentrations of propofol (50, 150, 250 mol/L) for 24 h enhanced MT-3 expression level after hypoxia/reoxygenation. When MT-3 expression was silenced by specific siRNA, propofol failed to improve hippocampal neuron cell survival rate.CONCLUSION: MT-3 exerts hippocampal neuron protective effect, which may possibly be mediated by the upreguation of MT-3 expression.

Key words: Propofol, Metallothionein-3, Hippocampal neuron cells, Hypoxia/reoxygenation

CLC Number:

R965.2

HE Jian-guo, HUANG Chang-shun, JIANG Juan, HU Ye, CAO Gang, LIU Zhen-wei. The impact of propofol pretreatment on hypoxia/reoxygenation in cultured hippocampal neurons metallothionein-3 protein expression[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(5): 517-520.

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