Expression of multidrug resistance-associated protein 1 in bronchial epithelium of models rat with chronic obstructive pulmonary disease and the reversal effects of Huatanjiangqi Capsule on it

Abstract

Abstract: AIM: To investigate the effects of Huatanjiangqi Capsule on the expression of multidrug resistance-associated protein 1 in the lung tissues of models rat with Chronic obstructive pulmonary disease (COPD). METHODS: COPD rat model was established by using smoke exposure composite papain aerosol inhalation. COPD rats were randomly divided into model group and Huatanjiangqi treatment group, and normal rats were as controls. Fifteen days after administration Huatanjiangqi, lung tissues were taken and expressions of MRP1 were determined by RT-PCR and Western blot. RESULTS: Huatanjiangqi Capsule could improve the lung function of COPD models rat. The MRP1 mRNA and protein expression in COPD model group was significantly lower than those in control group (P<0.01). Huatanjiangqi could up-regulate the MRP1 mRNA and protein expression compared with COPD model group (P<0.01). CONCLUSION: Huatanjiangqi Capsule could up-regulate the expression of MRP1 in the lung tissues of COPD models rat. This is probably the mechanism of Huatanjiangqi Capsule in treatment COPD.

Key words: COPD, MRP1, Expression, Huatanjiangqi Capsule, Reversal effect

CLC Number:

R965

WANG Dian-lei, ZHANG Xian, TAO Xiu-hua, CAO Yin, PENG Bo, LI Ze-geng. Expression of multidrug resistance-associated protein 1 in bronchial epithelium of models rat with chronic obstructive pulmonary disease and the reversal effects of Huatanjiangqi Capsule on it[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(6): 621-626.

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