Effect of carbachol and thymosin on the function of cell-ular immune in patients with multiple organ dysfunction

Abstract

Abstract: AIM: To study carbachol and thymosin on cellular immune function in patients with MODS. METHODS: 208 cases of MODS patients were selected, which were randomly divided into the normal treatment group, carbachol group, thymosin group and the combined treatment group. Using flow cytometry to test the changes of CD₁₄⁺ monocots human leukocyte antigen DR (HLA-DR) and Lymphocyte apoptosis. RESULTS: Normal treatment group was compared with the other treatment groups, the difference was statistically significant (P<0.05); carbachol group was compared with the thymosin group, the difference was not statistically significant (P>0.05); the combination therapy group comparing with other groups,the difference was also significant (P<0.05). Carbachol and thymosin can enhance immune function in patients with MODS, combined treatment doing better effect. CONCLUSION: Carbachol and thymosin has some clinical value on the treatment of MODS.

Key words: Carbachol, Thymosin, MODS, HLA-DR, Apoptosis

CLC Number:

R968

CHENG Ai-bin, SHANG Xiu-juan, WANG Rui-gang, DIAO Zeng-li, ZHANG Jun-wei. Effect of carbachol and thymosin on the function of cell-ular immune in patients with multiple organ dysfunction[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(6): 677-681.

References

[1] Lin RY, Astiz ME,Saxon JC, et al. Altered leukocyte immunophenotypes in septic shock:studies of HLADR, CD14 and IL-2 expression[J]. Chest,1995,104(3):847 - 853.
[2] Bone RC.Sir Isaac Newton;sepsis,SIRS and CARS[J].Crit Care Med,1996,24(7):1125-1128.
[3] Volk HD,ReinkeP,Krausch D. Monocyte dective ationrationale for a new therapeutic strategy in sepsis[J].Intensive Care Med,1996, 22(Suppl 4): S474-S481.
[4] Hershman MJ, Cheadle WG, Wellhausen SR, et al. Monocyte HLA-DR antigen expression characterizes clinical outcome in the trauma patient[J]. Br J Surg,1990,77(2): 204 -207.
[5] Effect of arginine on serum tumor necrosis factor receptor 1(sTNFR-1) and T lymphocytes rDNA transcription activity in peripheral blood of patients with pancreatic cancer after Intra -arterial che motherapy[J]. Herald of Medici ne, 2009,28(3):310-312.
[6] Unger SW, Crockard AD,Halliday MI,et al. Class Ⅱmajor histocompatibility comple antigen expression on peripheral blood monocytes in patients with inflammatory bowel disease[J]. Gut, 1994, 35(4): 511- 516.
[7] Borovikova LV, Ivanova S, Zhang M,et al. Vagus nerve stimulation attenuates the systemic inflammatory response to endotoxin [J]. Nature, 2000, 405(6785): 458-462.
[8] Young GB,Bohon CF,Austin TW. Definitions for sepsis and organfailure and guidelines for the use of innovative therapies [J]. Crit Care Med,1992,20(6):724-726.
[9] Cinel I, Buyukafsar K,Cinel L, et al. The role of poly(ADP-ribose)synthetase inhibition in preventing endotoxemia-induced intestinal epithelial apoptosis[J]. Phamacol Res, 2002, 46(2): 119-127.
[10] Aranow JS, Fink MP. Determinants of intestinal barrier failure in critical illness[J]. Br J Anaesth 1996, 77(1): 71-81.
[11] Berkes J, Viswanathan VK, Savkovic SD, et al. Intestinal epithelial responses to enteric pathogens: effects on the tight junction barrier, ion transport, and inflammation[J]. Gut, 2003, 52(3): 439-451.
[12] Hassoun HT,Kone BC,Mercer DW,et al. Post-injury multiple organ failure:the role of the gut[J]. Shock, 2001, 15(1): 1-10.
[13] Young GB,Bohon CF,Austin TW.Definitions for sepsis and organfailure and guidelines for the use of innovative therapies [J]. Crit Care Med, 1992, 20(6): 724-726.
[14] 程爱斌,邱方,安丽红,等. 胸腺肽对严重脓毒症患者细胞免疫功能的影响[J]. 江苏医药,2010,36(7):853-854.
[15] 林洪远,盛志勇. 脓毒症免疫调理治疗的新思路[J]. 中国危重病急救医学,2004,16(2):67-69.
[16] Jang XG, Hu S, Shi DG, et al. Effects of carbachol on plasma levels of tumor necrosis-a and interleukin-10 in intestinal ischemia-reperfusion rat [J]. Zhongguo Wei Zhong Bin Ji Jiu Yi Xue, 2003, 15(3): 167-169.
[19] Hata Y, Kawabe T, Hiraishi H, et al. Hydrogen peroxide-mediated cytotoxicity to cultured colonic epithelial cells[J]. Life Sci, 1997, 60(24): 2221-2230.