Protective effects of levocarnitine combined with bisoprolol on cardiopulmonary resuscitation in  rats

Abstract

Abstract: AIM: To investigate the L-carnitine and bisoprolol on myocardium after cardiopulmonary resuscitation in a synergistic protective effect and its mechanism.METHODS: Rats were randomly divided into 4 groups: L-carnitine and bisoprolol combined therapy group (10 cases), the bisoprolol group (10 cases), carnitine group (10 cases ), the placebo group (10 cases). Each rat heart rate and mean arterial pressure at different time points were observed. Myocardial injury markers CK-MB was detected by enzyme biochemical assay and serum oxidative stress indices SOD and MDA were detected by ELISA, and myocardial BCL-2 and Bax protein were detected by Western Blot method,and the levels of EtBr and 3-NT were tested.RESULTS: Compared with the bisoprolol group and the placebo group, L-carnitine group each time heart ratewas increased(P<0.05), mean arterial pressure was also increased(P<0.05), the levels of SOD and BCL-2 were increased, CK-MB, MDA and Bax levels were decreased(P<0.05). Compared with the placebo group, the levels of EtBr and 3-NT of L-carnitine group were decreased(P<0.05), while combination group compared with the placebo group, the differences were more significant (P<0.01).CONCLUSION: L-carnitine can upregulate SOD and BCL-2 levels, downregulate MDA and Bax levels to produce effect on myocardium, while bisoprolol has harmonious effect.

Key words: L-carnitine , Bisoprolol, Cardiopulmonary resuscitation, Myocardial apoptosis

CLC Number:

R965.2

ZHANG Mei-qi, ZHAI Chang-lin, CHEN Huan, SHEN Yue, TU Jian-feng, YANG Xiang-hong. Protective effects of levocarnitine combined with bisoprolol on cardiopulmonary resuscitation in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(12): 1321-1326.

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Protective effects of levocarnitine combined with bisoprolol on cardiopulmonary resuscitation in rats

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