Effects of different fractions extracted from Tupistra chinensis Bak on isoproterenol- induced myocardial injury in mice

Abstract

Abstract: AIM: To investigate the effects of different fractions extracted from Tupistra chinensis Bak on isoproterenol (ISO)-induced myocardial injury in mice.METHODS: 105 KM mice were evenly and randomly divided into normal group, model group, four different fractions extracted from Tupistra chinensis Bak groups and Di'aoxinxuekang group. The treatment groups were orally treated homologous medicines, the normal and model groups were given the same volume of 0.5% CMC-Na. After the treatment of 30 min on the subsequently 3 days, the model group, four different fractions extracted from Tupistra chinensis Bak groups and Di'aoxinxuekang group were injected subcutaneously with ISO (5 mg/kg), while the animals in normal group were received injection of normal saline. Blood samples from mice on the next day after the last administration were collected plasm levels of AST, LDH, HBDH, CK and CKMB. The mice were then sacrificed under anesthesia to collect myocardial tissues for measurements of heart weight index (heart weight/body and left ventricles weight/heart weight), myocardial SOD,GSH-Px activities and MDA content, and analysis of heart tissue morphology and ultra microstructure.RESULTS: The different fractions extracted from Tupistra chinensis Bak might significantly reversed the ISO-induced heart index, abnormal higher myocardial tissue MDA contents and less SOD, GSH-Px activities, and also reduced abnormal higher plasm AST, LDH, HBDH, CK and CKMB levels. The myocardial inflammatory infiltration, lytic necrosis, collagen fiber area were remarkably alleviated due to the four fractions. The effects of the n-butyl alcohol and ethyl acetate fractions were significant as compared with the model group (P＜0.05, P＜0.01), and the effect of treatment with Di'aoxinxuekang quite; Petroleum ether fraction revealed a statistically significant in improving HBDH and SOD levels compared with the model group (P＜0.05), and that of water fraction was also significant as compared with the model group except for in the case of LDH, CK and GSH-Px (P＜0.05).CONCLUSION: The treatments with different fractions extracted from Tupistra chinensis Bak have remarkably protective effect. The effects from strong to weak are as follows: the n-butyl alcohol fraction, ethyl acetate fraction, water fraction and petroleum ether fraction.

Key words: Tupistra chinensis Bak, Extract fraction, Isoproterenol, Myocardial ischemia

CLC Number:

R965.2

SHI Meng-qiong, BAI Cai-hong, SUN Gui-lin, JIN Jia-hong, YANG Wen-yan, LU Xun-cong. Effects of different fractions extracted from Tupistra chinensis Bak on isoproterenol- induced myocardial injury in mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(12): 1344-1352.

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