Effects of naloxone on the expressions of JNK3 protein in rats after ischemia-reperfusion

Abstract

Abstract: AIM: To investigate the effect of naloxone on the expression of JNK3 protein in Hippocampus during transient focal cerebral ischemia reperfusion, and to discuss the probable mechanism of its protective effect.METHODS: The model of focal cerebral ischemia-reperfusion injury was established in rats by suture-occluded method. Rats were randomly divided into several groups, including Sham-operation, ischemia-reperfusion, low dose naloxone treated ischemia-reperfusion, high dose naloxone treated schemia-reperfusion, SP600125 treated ischemia-reperfusion and respective Vehicle treated ischemia-reperfusion. The infarction volume was detected by TTC staining. Western Blot was operated to detect expression of p-JNK3 in Hippocampus.RESULTS: Both groups of high dose naloxone treated ischemia-reperfusion and SP600125 treated ischemia-reperfusion, not only ischemia-reperfusion infarction volume was reduced, but also expression of p-JNK3 was down-regulated, compared with ischemia-reperfusion only group. As the dose increase, the effect of naloxone on inhibiting expression of p-JNK3 was obviously improved.CONCLUSION: Naloxone inhibits the expression of p-JNK3 in a dose-dependent manner during focal ischemia reperfusion which may be one of the mechanisms of its neuroprotective function.

Key words: Naloxone, Cerebral ischemia-reperfusion, c-Jun N-terminal kinase 3

CLC Number:

R965.2

TIAN He-ping, CHU Zheng-min, JIN Cheng-shen, SHEN Jian-guo. Effects of naloxone on the expressions of JNK3 protein in rats after ischemia-reperfusion[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(3): 258-262.

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