Abstract: AIM: To study antitumor effect of extracellular polysaccharides from Trichoderma pseudokoningii.METHODS: In vitro, we studied the antitumor activity of TPeps on human leukemia HL-60 cells with MTT, and in vivo, TPeps were intragastrically given to mice with S180 solid tumor at the dose of 50, 100 and 200 mg·kg^-1·d^-1. Normal sodium and cyclophosphamide were taken as blank control and positive control respectively. The tumor weight, spleen index, thymus index and the relative growth rate were measured.RESULTS: TPeps could significantly inhibit HL-60 cells proliferation in a time and concentration dependent manner. Meanwhile, compared with control TPeps three dose groups significantly decreased the weight of tumor, increased the spleen index and thymus index(P<0.05,P<0.01). However, TPeps had no discernible effect on the relative growth rate.CONCLUSION: TPeps have a certain amount of anti-tumor action.

Key words: Trichoderma pseudokoningii, Exopolysaccharides, Antitumor effect, Human leukemia HL-60 cells, Sarcoma-180