Treatment of artesunate on the mouse model of acute pancreatitis induced by combining cerulean and lipopolysaccharide

Abstract

Abstract: AIM: To evaluate the effect of artesunate (AS) on rats with acute pancreatitis. METHODS: Sixty Kunming mice were randomly divided into five groups: control group, AP induction group and treatment group with AS. Then the treatment group was divided into three subgroups according to the concentration of AS. In the AP induction group and AS treatment group, cerulean (100 μg/kg) was intraperitoneal injected into animals every one hour for six times, then intraperitoneal injections of LPS (10 mg/kg). In the AS treatment group, AS was intramuscular injected at zero and four hours after the induction of pancreatitis. The animals were sacrificed at the 18 h hour after the first intraperitoneal injection. The levels of AMS, LPS, trypsin, SOD in serum were determined. HE staining was performed to evaluate the morphologic changes in animal pancreats. RESULTS: The amount of AMS, LPS and trypsin in serum and the pancreatic injury severity in AS treatment group were markedly lower than those in the AP induction group(P<0.05 or P<0.01). The amount of SOD was just the opposite. CONCLUSION: AS might ameliorate AP in this mouse model.

Key words: acute pancreatitis, artesunate, serum amylase, superoxide dismutase

CLC Number:

R965.2

YAN Zi-fei, CEN Yan-yan, LI Xiao-li, KUANG Mei, CAO Guan-qun, LI Pan, ZHOU Hong. Treatment of artesunate on the mouse model of acute pancreatitis induced by combining cerulean and lipopolysaccharide[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(4): 361-365.

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