Down-regulation of hyppocampal TH and TPH expression involved in CUMS-induced depressive behaviors of rats and interfering effects of venlafaxine

Abstract

Abstract: AIM: To investigate if chronic unpredictable mild stress (CUMS)-induced anxiety-depression-like behaviors of rats is associated with the expression changes of hyppocampal TH and TPH and interfering effects of venlafaxine. METHODS: Sixty-four male SD rats were randomly divided into normal group (NG), model group (MG), Venlafaxine -treated normal group (WNG), and Venlafaxine -treated model group (WMG).CUMS with solitary condition was taken to establish rat depression model.The open field test (OFT) and Forced swim test (FST) were used to evaluate depressive behaviors, biochemistry kit, real-time PCR, and western blot were used to determine SOD activity and MDA level of cortex, as well as mRNA and protein expressions of hippocampus TPH and TH. RESULTS: Compared with corresponding control, the open field score (ambulation and rearing), cortex SOD activity, as well as mRNA and protein expression of hippocampus TPH and TH significantly were decreased with obviously increasing cortex MDA and immobility time of FST in MG. The treatment of Venlafaxine obviously blunted the changes above caused by CUMS, but failed to affect normal rats. CONCLUSION: CUMS-induced depression of rats is associated with down-regulation of hyppocampal TH and TPH expression, which can be blunted by administration of Venlafaxine.

Key words: depression, CUMS, venlafaxine, TPH, TH

CLC Number:

QIN Li-juan, LIU Dan, JIANG Xing-hui, QIU Hong-mei, WANG Li-jia, LIU Ren, FEI Hui-zhi, HU Xiao-ya, ZHOU Qi-xin. Down-regulation of hyppocampal TH and TPH expression involved in CUMS-induced depressive behaviors of rats and interfering effects of venlafaxine[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(4): 398-403.

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