Effects of siRNA silencing COX-2 gene on VEGF-C expression and cell migration in SGC-7901 cells

Abstract

Abstract: AIM: To investigate the effects of using RNA interference to silence the expression of COX-2 gene on the expression of VEGF-C and cell migration. METHODS: Using siRNA interference the COX-2 gene in gastric carcinoma cell line SGC-7901, and RT-PCR and immunofluorescence detect the expression of COX-2 and VEGF-C gene,comparison the effect COX-2 gene on cell migration by cell scratch test.RESULTS: COX-2 gene and VEGF-C gene were significantly reduced in mRNA and protein levels after the silence of COX-2 gene in gastric cancer cell line SGC-7901,with statistically significant difference between groups (P<0.05). The expression of COX-2 was closely correlated with the expression of VEGF-C(P<0.05); the migration ability of gastric cancer cells also decreased. CONCLUSION: In gastric carcinoma cell SGC-7901, siRNA can inhibit the COX-2 expression,and COX-2 gene could enhance the migration ability of gastric cancer cells.The silence of COX-2 gene could download the expression of VEGF-C,reduce the migration ability of gastric cancer cells, therefore reducing the metastasis of gastric cancer cell.

Key words: gastric carcinoma, RNA interference, COX-2, VEGF-C, cell migration

CLC Number:

R965.2

LUO Hui-ying, YANG Lin, WANG Xiao-peng, SU He, LIU Ying, MA Yun-tao, WU Zi-yan. Effects of siRNA silencing COX-2 gene on VEGF-C expression and cell migration in SGC-7901 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(9): 961-966.

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