Effect of methotrexate on spinal cord contusion-induced protein related with oxidative damage in rats at acute stage

Abstract

Abstract: AIM: To examine the effect of methotrexate on spinal cord contusion-induced protein related with oxidative damage in rats at acute stage and to explore its neuroprotective mechanism. METHODS: Rat spinal cord contusion model was duplicated by a BenchmarkTM stereotaxic cortical impactor which connected with PinPoint? precision contact sensor. At posttraumatic 30 min, Treatment group was subcutaneously administrated methotrexate (0.5 mg/kg, according to the weight),Control group and Sham group were injected the same volume of physiological saline. At posttraumatic 1,3,6,12,24,48,72 h, blood and tissue samples were collected. ELISA method was used to determine the content of 3-nitrotyrosine (3-NT) in plasma,advanced oxidation protein products (AOPP) and protein carbonyl in injuried tissue. RESULTS: At all the time point after surgery, the contents of 3-NT, AOPP and protein carbonyl in Treatment group were lower than those in Control group. Post-traumatic 12h to 72 h, the contents of 3-NT in Treatment group were significantly lower than those in Control group (P<0.05). Post-traumatic 24 h to 72 h, the contents of AOPP in Treatment group were significantly lower than those in Control group (P<0.05). However, the content of protein carbonyl at each time point had no statistical significance between the two groups. CONCLUSION: The role of Methotrexate in preventing the secondary spinal cord injury may be related to reducing the formation of protein related with oxidative damage.

Key words: methotrexate, traumatic spinal cord injury, acute phase, 3-nitrotyrosine, advanced oxidation protein products, protein carbonyl

CLC Number:

ZHANG Si, GU Bing, LI Hua-nan, WANG Shuo-yu, ZHANG Shui-yin. Effect of methotrexate on spinal cord contusion-induced protein related with oxidative damage in rats at acute stage[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(1): 1-6.

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