Mechanism investigation of low dose ethanol intervention on myocardial fibrosis in diabetic rats

Abstract

Abstract: AIM: To study the effects of low dose ethanol intervention on myocardial fibrosis in diabetic rats and analyze its underlying mechanism.METHODS: Male SD rats were randomly divided into four groups: normal control group (Con), diabetes at 4th week group (DM 4w), diabetes at 8th week group (DM 8w) and ethanol + diabetes at 8th week group (EtOH+DM 8w). After 8 weeks, the hemodynamic parameters were recorded, heart weight/body weight ratio and hydroxyproline content in myocardium were measured. The changes of collagen morphology in myocardial tissue were observed with Masson staining, collagen volume fraction (CVF) was measured by image analysis. The expression of ALDH2 at mRNA level of myocardium were detected by Real-Time PCR analysis, and the protein expressions of p-JNK and JNK were detected by Western blot.RESULTS: In contrast with Con group, there were no differences about hemodynamic parameters in DM 4w group, but in DM 8w group, mean arterial pressure and heart rate were decreased, the ratio of heart weight/body weight, hydroxyproline content and CVF were markedly increased, ALDH2 mRNA expression was decreased, while the ratio of p-JNK/JNK were increased. Compared with DM 8W group, the above indexes were improved in EtOH+DM 8w group.CONCLUSION: Low dose ethanol ameliorated myocardial fibrosis in diabetic rats by up-regulating ALDH2 expression and down-regulating JNK expression.

Key words: diabetes, myocardial fibrosis, aldehyde dehydrogenase 2, JNK, ethanol

CLC Number:

YU Ying, ZHANG Guan-jun, ZONG Qiao-feng, WANG Xiao-mei, LI Zhen-hong, GAO Qin. Mechanism investigation of low dose ethanol intervention on myocardial fibrosis in diabetic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(4): 379-383.

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