Relationship between related factors of canonical Wnt pathways and colorectal cancer staging

Abstract

Abstract: AIM: To evaluate the relationship between the related factors of Wnt/β-catenin signaling pathways and the stages of colorectal cancer.METHODS: We collected the clinical data, serum specimens and pathological tissue samples of 46 cases of patients with colorectal cancer who admitted in gastrointestinal surgery of the first affiliated hospital of Wannan Medical college from January 1, 2013 to January 1, 2014. According to the Dukes staging, these was divided into four groups. 10 cases of normal intestinal mucosa specimens were recruited as control group 1. The protein expression of Wnt-2, β-catenin, Axin, APC were detected by using immunohistochemical methods. 15 serum samples of healthy individuals were recruited as control group 2. The concentration of Wnt-2, β-catenin, Axin, APC in serum were detected by using enzyme-linked immunosorbent assay.RESULTS: The protein expression of Wnt-2 and β-catenin in colorectal cancer tissue were significantly higher than that in the normal intestinal mucosa, while the Axin and APC were lower than that in the normal intestinal mucosa, and the differences between different colorectal cancer stagings were statistically significant. The concentration of Wnt-2 in 46 cases of colorectal cancer patients serum was significantly higher than that in healthy people, and the differences between different colorectal cancer staging have statistical significances. The serum concentration of Axin was lower than in the healthy people, but there was no statistical difference between different colorectal cancer stagings.CONCLUSION: The expression of the related factors of Wnt/β-catenin signaling pathways was associated with colorectal cancer Dukes staging. The related factors of Wnt/β-catenin signaling pathways can be used as auxiliary serum examination index, contributing to improve colorectal cancer detection sensitivity.

Key words: canonical Wnt pathways, colorectal cancer, Wnt-2, β-catenin, Axin, APC

CLC Number:

TAO Kun, ZHAO Guo-hai, ZHANG Yi-sheng, XU Hua-ping, CHEN Fang-zheng, ZHANG Xiao-feng, WU Ze-hui. Relationship between related factors of canonical Wnt pathways and colorectal cancer staging[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(4): 414-419.

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