Effects of Rutin combined with oxaliplatin on the proliferation and apoptosis of human gastric cancer SGC-7901 Cells

Abstract

Abstract:

AIM: To study the effect of rutin combined with oxaliplatin on the proliferation and apoptosis of human gastric cancer SGC-7901 cells. METHODS: Human gastric cancer SGC-7901 cells in logarithmic growth phase were selected as the research object.Hoechst33258 nuclear staining was used to observe the morphological changes. Cell cycle and cell apoptosis were detected by flow cytometry. The expression of caspase-3, Bax and Bcl-2 protein in SGC-7901 cells were analyzed by Western-blot. RESULTS: Rutin (100,200,300 μmol/L) and oxaliplatin inhibited the proliferation of SGC-7901 in a dose-dependent manner. Rutin combined with oxaliplatin, compared with the same concentration of oxaliplatin SGC-7901 cells, was significantly more effective on SGC-7901 cells (P<0.05). Compared with oxaliplatin, SGC-7901 cells treated with different concentrations of rutin and oxaliplatin significantly increased the apoptosis rate of SGC-7901 cells (P<0.05).The ratio of bcl-2/bax protein expression was significantly decreased (P<0.05). CONCLUSION: Both curcumin and oxaliplatin can inhibit the proliferation of gastric carcer cells SGC-7901 and induce apoptosis. The mechanism may be related to up-regulation of caspase-3 and down-regulation of apoptosis-related protein such as bcl-2/bax. And the joint application of rutin and oxaliplatin presents some synergistic effect.

Key words: rutin, oxaliplatin, gastric cancer, proliferation, apoptosis

CLC Number:

R965.2

LI Qi, REN Liqun, WANG Yadi, CHEN Suxian. Effects of Rutin combined with oxaliplatin on the proliferation and apoptosis of human gastric cancer SGC-7901 Cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(10): 1099-1105.

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