Protective effects of Longxuetongluo capsule on cerebral ischemia injury in rats

Abstract

Abstract:

AIM: To investigate the protective effects of Longxuetongluo capsule on cerebral ischemia injury in rats. METHODS: The focal cerebral ischemia model was established to observe the extent of the pathological alteration and the focal cerebral ischemia reperfusion model was established to observe the change of behavior in rats. Meanwhile, the influence of Longxuetongluo capsule on the levels of Glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), lactate dehydrogenase (LDH), Malondialdehyde (MDA), NO and Induced nitric oxide synthase (iNOS) were investigated. RESULTS: Compared with model group, Longxuetongluo capsule of 0.66,0.33g/kg ameliorated neurologic deficits score and reduced the water content of brain tissue. In addition, Longxuetongluo capsule of 0.66 g/kg, 0.33g/kg can remarkly increased the levels of GSH-Px, SOD, LDH and decreased the activity of iNOS and the contents of NO and MDA. CONCLUSION: Longxuetongluo capsule has profective effects on cerebral ischemia injury and the mechanism maybe related to the resistance of free radical damage and the inhibiton of iNOS.

Key words: Longxuetongluo capsule, cerebral ischemia, SOD, NO, iNOS

CLC Number:

R965.2

LI Rui, LUO Shengyong. Protective effects of Longxuetongluo capsule on cerebral ischemia injury in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(11): 1232-1236.

References

［1］Namjoyan F, Kiashi F, Moosavi ZB, et al. Efficacy of Dragon's blood cream on wound healing: A randomized, double-blind, placebo-controlled clinical trial ［J］. J Tradit Complement Med, 2016, 6(1)：37-40.
［2］方伟蓉，李运曼，邓嘉元. 龙血竭总黄酮对动物心肌缺血的保护作用［J］. 中国临床药理学与治疗学，2005，10(9)：1020-1024.
［3］邹毅清，蔡志扬，李小宝，等. 红景天苷预处理对大鼠全脑缺血再灌注后炎症反应的影响［J］. 现代中西医结合杂志，2013，31(6)：253-255.
［4］曹泽标，周小青，刘旺华. 丹龙醒脑方对脑缺血再灌注损伤大鼠SVZ区Frizzled3、Dsh1及β-catenin表达的影响与促进神经干细胞增殖的机制［J］. 中华中医药杂志，2016, 31(10)：4206-4210.
［5］樊开宇，王冰，梅骁乐，等. 滁菊总黄酮对大鼠脑缺血再灌注损伤的保护作用［J］. 中国临床药理学与治疗学，2017，22(1): 9-12.
［6］Ran Y，Wang R，Gao Q，et al. Dragon's blood and its extracts attenuate radiation-induced oxidative stress in mice ［J］. J Radiat Res，2014, 55(4)：699-706.
［7］邓嘉元，李运曼，方伟蓉，等. 龙血竭总黄酮对乳鼠损伤心肌细胞的保护作用［J］. 中国天然药物，2006，4(5)：373-376.
［8］孔婧，陈婷，王爽，等. 活血通络清脑饮对老年短暂性脑缺血发作患者脑血流动力及纤维蛋白原的影响［J］. 长春中医药大学学报，2016，32(2)：335-336.
［9］陈春光，贾洪丽，吕首旭. 苦碟子注射液对大鼠急性脑缺血-再灌注损伤的保护作用［J］. 中国临床药理学杂志，2012，28(3)：196-199.
［10］王欣，施蝉宏，毛蓉嫣. 依达拉奉对老年短暂性脑缺血发作患者血清超氧化物歧化酶和丙二醛的影响［J］. 中华老年医学杂志，2015，34(12)：1310-1312.
［11］刘金莹，唐燕，王洪鑫，等. 利脑心胶囊对局灶性脑缺血模型大鼠血浆和脑组织中SOD、MDA含量的影响［J］. 长春中医药大学学报，2012，28(3)：400-401.
［12］王梅，胡晓彤，戚大石，等. 脑缺血/再灌注通过神经性一氧化氮合酶诱导KA受体亚基GluR6巯基亚硝基化的研究［J］. 中国当代医药，2015，9(27)：149-152.
［13］Chang Y，Chang TC，Lee JJ. Sanguis draconis, a dragon's blood resin, attenuates high glucose-induced oxidative stress and endothelial dysfunction in human umbilical vein endothelial cells ［J］. Scientific World J，2014，2014：1-10.

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