Effects of dexmedetomidine on JNK in A549 cells with hypoxia/reoxygenation injury

Abstract

Abstract:

AIM: To investigate the effects and c-Jun N-terminal kinase(JNK) expression of dexmedetomidine (DEX) on A549 cells with hypoxia/reoxygenation（H/R） injury. METHODS: A549 cells were cultivated and were randomly divided into four groups (n=10): control group (N), DEX group (D), hypoxia/reoxygenation injury group (H), hypoxia/reoxygenation injury+DEX interfere group (HD). After all models were completed, the morphological changes of A549 cells were observed under the inverted microscope. Cell activity was detected by CCK-8 and the apoptosis index (AI) was detected by in situ end labeling (TUNEL) method. The expression of GRP78, p-JNK, caspase-3 at protein levels and GRP78, JNK mRNA were detected by Western blot and RT-PCR.RESULTS:Compared with N group, the number of adherent cells in H group decreased significantly, and cell morphology changed. The expression of OD value in H group decreased obviously (P<0.01), the expression of AI value, GRP78, p-JNK, caspase-3 protein and GRP78, JNK mRNA were significantly increased (P<0.01). HD group compared with H group, the cell damage alleviated, the expression of OD value was increased (P<0.01), the number of apoptosis cells and the AI value in HD group were significantly decreased (P<0.01). Dramatically decreased the expression of p-JNK, caspase-3 protein and JNK mRNA (P<0.01). CONCLUSION: DEX can effectively alleviate A549 cells damage induced by H/R injury, which may be related to inhibition of the JNK pathway.

Key words: JNK, dexmedetomidine, hypoxia/reoxygenation injury, A549 cells, cell apoptosis

CLC Number:

R965.2

LUO Ziyin, XIANG Bingqian, GAO Hui, QIU Xiaoxiao, QIAN Xiaoying, WANG Wantie. Effects of dexmedetomidine on JNK in A549 cells with hypoxia/reoxygenation injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(12): 1346-1351.

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