Protective effect of total flavones of abelmoschl manihot on hippocampal neurons subjected to anoxia-reoxygenation injury

Abstract

Abstract:

AIM: To study the protective effect of total flavones of abelmoschl manihot (TFA) on hippocampal neurons subjected to the injury of anoxia/re-oxygenation (A/R) and its endothelium-dependent hyperpolarizing factor (EDHF) mechanism. METHODS: The injury of primarily cultured hippocampal neurons plus with cerebral basilar artery ring was induced by hypoxia for 4 hours and re-oxygenation for 24 hours, pretreated with TFA, acetylcholine (Ach),etc. Cell viability and the lactate dehydrogenase (LDH) activity in supernatant were served as the cell damage index. The level of free calcium fluorescence intensity in the neurons was detected by laser scanning confocal microscope. RESULTS: Pretreated with TFA and cerebral artery could increase the cell viability, reduce the activity of LDH in culture medium significantly, pretreated with Ach had a similiar effect. The N -nitro-L-arginine-methyl-ester (L-NAME) and indomethacin (Indo) didn't influence the effect of Ach or TFA. Moreover, pretreated with TFA plus with cerebral artery lowered the calcium concentration in neuron (compared with model group). CONCLUSION:TFA has remarkably neuroprotective effect induced by A/R, the mechanism is partially concerned with the calcium overload inhibited by EDHF released from cerebral basilar artery ring.

Key words: TFA, EDHF, A/R injury, LDH, calcium concentration

CLC Number:

R965.2

WEN Jiyue, CHEN Zhiwu. Protective effect of total flavones of abelmoschl manihot on hippocampal neurons subjected to anoxia-reoxygenation injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(4): 367-372.

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