Effect of Baishile capsule on expressions of BDNF，NT-3，NGF in septo-temporal axis of hippocampus of depressive rats

Abstract

Abstract:

AIM: To investigate the effect of Baishile capsule on the expression of neurotrophic factor including brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3), and nerve growth factor (NGF) in hippocampal septo-temporal axis of depressive rats. METHODS: Sixty SD rats were randomly divided into control group, model group of depression, fluoxetine group, high (2.88 g/kg), medium (1.44 g/kg) and low (0.72 g/kg) doses of Baishile capsule. Chronic unpredictable mild stress for 21 d was used to establish depression model; sucrose preferences and forced swimming test to detect depression-like behavior in rats; Morris water maze test to evaluate learning and memory ability; immunofluorescence technique to observe the content change of neurotrophic factors (BDNF,NT-3,NGF) in hippocampal septo-temporal axis of rats. RESULTS: Compared with the control group, sucrose preference was decreased while immobility time was increased significantly (P<0.01); escape latency and latency time in target quadrant presented a significant increase (P<0.01); times of crossing the target quadrant were significantly reduced (P<0.05); BDNF, NT-3, NGF expressions were down-regulated in S1, S2, T3 and T4 parts of hippocampal septo-temporal axis in model rats (P<0.01). Compared with model group, Baishile capsule could significantly improve sucrose preference degree and decrease immobility time (P<0.05); escape latency and latency time in target quadrant were significantly shorter (P<0.05); times of crossing the target quadrant increased in rats after administration of Baishile capsule (P<0.05); BDNF, NT-3, NGF expressions increased in S2 and T3 parts of septo-temporal axis in depressive rats (P<0.05 or P<0.01). CONCLUSION: Baishile capsule could significantly improve the depression-like behavior, learning and memory ability in depressive rats, which might be associated with regulating the function of S2, T3 parts of hippocampal septo-temporal axis, and promoting the expression of neurotrophic factors.

Key words: Baishile capsule, depression, chronic stress, hippocampus, septo-temporal axis, neurotrophic factor

CLC Number:

R965.2

MENG Pan, LIU Zhuo, ZHU Qing, ZHAO Hongqing, ZHANG Xiuli, LEI Chang, WANG Yuhong, YANG Hui. Effect of Baishile capsule on expressions of BDNF，NT-3，NGF in septo-temporal axis of hippocampus of depressive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(4): 418-423.

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