Correlation between cerebral blood flow and infarct volume of the middle cerebral artery occlusion in mice

Abstract

Abstract:

AIM: To analyze the correlation between the two evaluation methods of middle cerebral artery occlusion and focal cerebral ischemia so as to explore a novel method and standard for the evaluation of the model. METHODS: Twenty adult male kunming mice were randomly divided into the model group and the sham-operated group（n=10）.The focal cerebral ischemia reperfusion model was established by thread embolism method, and the changes of cerebral blood flow in each group were detected beforeischemia, the moment of ischemia, two hours after ischemia, immediately and 24 h after reperfusion. The rating system was employed to evaluate neurological function of mice at 24 h after ischemia reperfusion, the brain infract volume percentage was assessed after 2% TTC staining following the last neurological evaluation,and the correlation between the infarct volume and cerebral blood flow ratio was analyzed by correlation analysis. RESULTS:Compared with sham group, the mice in the model group exhibited obvious neurological abnormalities, the TTC staining showed obvious infarction, and the cerebral blood flow of the infarct side was significantly decreased（P<0.05). The correlation coefficient of the cerebral blood flow and the infarct volume at 24 h after reperfusion were 0.55 and 0.28（P<0.05）,respectively. CONCLUSION: There was a high linear correlation between cerebral infarct volume at 24 h reperfusion and cerebral blood flow immediately after ischemia in model group. Cerebral blood flow is a reliable indicator to confirm the establishment of mouse MCAO model besides monitoring theneurological function evaluation and infarct volume.

Key words: focal cerebral ischemia reperfusion model, neurological function evaluation, cerebral infarction volume, cerebral blood flow

CLC Number:

R965.2

XIE Yuewu, ZHANG Fang, GUO Yan. Correlation between cerebral blood flow and infarct volume of the middle cerebral artery occlusion in mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(5): 501-506.

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