Antitumoral effects of Sijunzi decoction on side population of SGC-7901 in nude mice and mechanism

doi:10.12092/j.issn.1009-2501.2018.05.004

Abstract

Abstract:

AIM: To investigate the antitumor effect of Sijunzi decoction on side population (SP) cells and non-side population (NSP) cells of gastric cancer cell line SGC7901 in nude mice. METHODS: Human gastric cancer cell line SGC-7901 SP cells were conventionally cultured, then, SP and NSP cells were sorted by flow cytometry. Proportion of SP cells was about 1.9%. The model of tumor-bearing mice was established and drug intervention group mice were given a gavage of Sijunzi decoction. All mice were killed after 30 days and the tumor growth curve was drawn and the tumor growth inhibition rate was measured. Tumor growth inhibition rate (IR) was calculated and apoptosis was detected by TUNEL method. RESULTS:The mean tumor volumes of SP-Drug group, NSP-Drug group were (1 348.40±103.98) mm³, (628.99±98.87) mm³, and their model group were (3 307.76±491.44) mm³, (2 202.87±67.80) mm³ (P＜0.05), respectively. Tumor inhibition rate of SP-drug group and NSP-drug group were 54.69% and 60.19%, which were significantly different from the control groups (P＜0.05). There was also a difference between SP group and the NSP group (P＜0.05). TUNEL results showed that there were more apoptotic cells in the drug groups. The apoptosis rate of SP group and NSP group were 20.21% and 16.53%, which were obviously higher than control groups (P＜0.05). CONCLUSION: Sijunzi decoction can inhibit the growth of human gastric cancer SGC-7901 SP cells in nude mice in vivo, which was probably related to induction of apoptosis in tumor cells.

Key words: Sijunzi decoction, gastric cancer, side population cell, apoptosis

CLC Number:

R965.2

CHEN Shaohua, LI Nan, QIAN Jun. Antitumoral effects of Sijunzi decoction on side population of SGC-7901 in nude mice and mechanism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(5): 504-509.

References

［1］王秀薇, 王少墨, 柳涛, 等. 王庆其中医药治疗胃癌前病变的思路探讨［J］. 辽宁中医杂志, 2015(2): 253-255.
［2］Meyer HJ, Wilke H. Gastric cancer: current status of multimodality treatment［J］. Zentralbl Chir, 2011, 136(4): 317-324.
［3］Dikken JL, van de Velde CJ, Coit DG, et al. Treatment of resectable gastric cancer［J］. Thera Adv Gastroenterol, 2012, 5(1): 49-69.
［4］张建平, 杨丽娟, 赵会颖, 等. 核仁素和骨形态发生蛋白-2在胃癌细胞上皮间质转化中的作用［J］. 疑难病杂志, 2017, 16(7): 713-716.
［5］王克穷, 张振兴, 张天瑶. 四君子汤对胃癌细胞植入小鼠术后复发细胞内VEGF及生存期的影响［J］. 福建中医药, 2012, 43(1): 43-44.
［6］Lian Y, Xie L, Chen M, et al. Effects of an astragalus polysaccharide and rhein combination on apoptosis in rats with chronic renal failure［J］. Evid Based Complement Alternat Med: eCAM, 2014, 2014(3): 291-299.
［7］Goodell MA, Brose K, Paradis G, et al. Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo ［J］. J Exp Med, 1996, 183(4): 1797-1806.
［8］Wu CP, Zhou L, Xie M, et al. Identification of cancer stem-like side population cells in purified primary cultured human laryngeal squamous cell carcinoma epithelia［J］. PLoS One, 2013, 8(6): e65750.
［9］He K, Xu T, Xu Y, et al. Cancer cells acquire a drug resistant, highly tumorigenic, cancer stem-like phenotype through modulation of the PI3K/Akt/beta-catenin/CBP pathway［J］. Int J Cancer, 2014, 134(1): 43-54.
［10］Teshima K, Nara M, Watanabe A, et al. Dysregulation of BMI1 and microRNA-16 collaborate to enhance an anti-apoptotic potential in the side population of refractory mantle cell lymphoma［J］. Oncogene, 2013, 33(17): 2191-2203.
［11］张闻多, 丁文惠. 细胞凋亡与凋亡诱导因子［J］. 中国病理生理杂志, 2006, 10(9): 1858-1862.
［12］杨婕, 姚敏, 王理, 等. 干预磷脂酰肌醇蛋白多糖3基因转录联合抗肿瘤药物抑制肝癌细胞增殖的协同作用［J］. 临床肝胆病杂志, 2016, 32(12): 2347-2352.
［13］林明生, 王常松. 中医药对胃癌术后化疗患者的辨治思路［J］. 长春中医药大学学报, 2015, 31(2): 278-280.
［14］黄浏姣, 洪亚群, 赵刚. 四君子汤含药血清与氟尿嘧啶合用对胃癌细胞SGC-7901的生长抑制作用［J］. 湖北中医药大学学报, 2011, 13(6): 3-5.
［15］朱超, 刘瑞, 郭晨旭, 等. 四君子汤及其拆方对胃癌SGC7901侧群细胞增殖、侵袭能力的影响［J］. 中国临床药理学与治疗学, 2015, 20(11): 1201-1207.
［16］Cheng L, Luo, Xi P, et al. Comparison of labeling methods and time course of traumatic brain injury-induced cell death in mice［J］. Neural Regen Res, 2010, 5(9): 706-709.

[1]	FAN Jie, JIN Yongming, JIANG Xiaolong, JIANG Guohua. Molecular mechanism of lncRNA HOTAIR regulating miR-206 on the proliferation and apoptosis of rheumatoid arthritis synovial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 736-742.
[2]	YANG Xiaojia, JIANG Meng, WU Min, ZHANG Yili, LV Lan, WU Yuanfen, WANG Xinyu, LIU Liquan. Improvement effect of crocin on cognitive impairment of Alzheimer's disease rats through DKK3 regulation of GSK-3 β/β-Catenin pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 489-497.
[3]	WANG Yan, ZHANG Jinhui, ZHAO Yongchen, LIU Hongxiang, LIU Yawei, MIAO Huanhuan, YANG Xincai. Sequoiaflavone inhibits stem cell properties such as proliferation and invasion of gastric cancer cells by down-regulating PI3K/AKT signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 508-513.
[4]	ZHANG Xuejiao, LIU Jieting, LI Luxin, CHEN Peijian, DING Minglu, SUN Mengwei, CHU Yanhui, ZHANG Zhen . Effects and mechanism of dapagliflozin on myocardial injury in type 1 diabetes mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(3): 257-265.
[5]	HOU Xiaoyu, LENG Yufang, CAO Xuefen, LV Xingjiao, HAN Xiaoxia, Janvier NIBARUTA, LIU Yongqiang. Network analysis and experimental verification of Schisandrin B reduces intestinal ischemia reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(2): 147-154.
[6]	WANG Dan, YAN Xiaoli, ZHANG Yuan. Helicobacter pylori infection influences the efficacy of immunotherapies for gastric cancer#br# [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(2): 228-234.
[7]	WANG Jian, SUN Yongdong, ZHOU Xingwei, LIU Lei, CHEN Long, TONG Xingke, ZHU Jiali. Baicalein promotes laryngeal cancer cell death and inhibits invasion via miR-125b-5p/IRF4 axis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1209-1218.
[8]	ZHU Ruifang, GUO Dongkai, ZHI Hui, JIANG Yiguo, ZHANG Yueling, QIAN Xiaoping, JI Shiliang. Exploring the mechanism of elemene synergistic bortezomib against multiple myeloma by ROS-NF-κB-p38MAPK pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1219-1226.
[9]	ZHOU Huan, XU Ming, LI Bo, LIU Chunying, WANG Chun. PI3K/Akt/FOXO3a signaling pathway inducing protective autophagy promotes acquired lung adenocarcinoma resistance remodeling to DDP [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(9): 961-970.
[10]	HUA Haiyan, YAN Jie, QIN Yufen. Experimental study of schisandrin B attenuates acute myocardial ischemia injury in rats by inhibiting cardiomyocyte apoptosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 848-856.
[11]	LI Xueheng, LI Long. Research progress on the relationship between melatonin and idiopathic pulmonary fibrosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(6): 715-720.
[12]	YANG Liu, LUO Ailin, LI Shiyong. Advance of general anesthetics-induced developing brain injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(12): 1381-1390.
[13]	TAN Fenglang, REN Hongli, WANG Hongfei. Effect of Phellodendri amurense extract on hippocampal nerve cells induced by Aβ_1-42 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1213-1220.
[14]	PAN Rongrong, ZHI Yinghao, JIN Yongxi, ZHOU Xiahui. Influences of theaflavins on neuronal apoptosis and blood-brain barrier in rats with cerebral hemorrhage by regulating CaMKK2/AMPK signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1240-1246.
[15]	LI Ying, YAO Wei, WANG Meng, YU Zhihong, GONG Yuanqi, LAN Haibing, QI Xiefei. Experimental study of irisin alleviates house dust mite-induced airway epithelial cells inflammation and apoptosis via the NF-κB and JNK signaling pathways [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(10): 1106-1112.