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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2018, Vol. 23 ›› Issue (5): 504-509.doi: 10.12092/j.issn.1009-2501.2018.05.004

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Antitumoral effects of Sijunzi decoction on side population of SGC-7901 in nude mice and mechanism

CHEN Shaohua, LI Nan, QIAN Jun   

  1. Department of Pathology, First Affiliated Hospital of Bengbu Medical College, Department of Pathology, Bengbu Medical College, Bengbu 233004, Anhui, China
  • Received:2017-10-16 Revised:2017-12-28 Online:2018-05-26 Published:2018-05-16

Abstract:

AIM: To investigate the antitumor effect of Sijunzi decoction on side population (SP) cells and non-side population (NSP) cells of gastric cancer cell line SGC7901 in nude mice.  METHODS: Human gastric cancer cell line SGC-7901 SP cells were conventionally cultured, then, SP and NSP cells were sorted by flow cytometry. Proportion of SP cells was about 1.9%. The model of tumor-bearing mice was established and drug intervention group mice were given a gavage of Sijunzi decoction. All mice were killed after 30 days and the tumor growth curve was drawn and the tumor growth inhibition rate was measured. Tumor growth inhibition rate (IR) was calculated and apoptosis was detected by TUNEL method. RESULTS:The mean tumor volumes of SP-Drug group, NSP-Drug group were (1 348.40±103.98) mm3, (628.99±98.87) mm3, and their model group were (3 307.76±491.44) mm3, (2 202.87±67.80) mm3 (P<0.05), respectively. Tumor inhibition rate of SP-drug group and NSP-drug group were 54.69% and 60.19%, which were significantly different from the control groups (P<0.05). There was also a difference between SP group and the NSP group (P<0.05). TUNEL results showed that there were more apoptotic cells in the drug groups. The apoptosis rate of SP group and NSP group were 20.21% and 16.53%, which were obviously higher than control groups (P<0.05). CONCLUSION: Sijunzi decoction can inhibit the growth of human gastric cancer SGC-7901 SP cells in nude mice in vivo, which was probably related to induction of apoptosis in tumor cells.

Key words: Sijunzi decoction, gastric cancer, side population cell, apoptosis

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